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How do you separate two different proteins of same molecular weight?
Wiki User
∙ 13y ago
that two proteins having different PI value . so run that two proteins in capillary having different PI regions. then they can separate easily according to their PI value.
Wiki User
∙ 13y ago
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Does a protein solution have a high molarity?
No. The reason is that low molecular weight compounds tend to have high molarity. As an example NaF is 42.5 molecular weight. So 42.5g dissolved in 1 liter of water would only be 4.25% but be 1 molar. Proteins tend to have every high molecular weight. So if a protein was say 1000 molecular weight, a 10% solution would contain 100g but only be 0.1 molar.
Why native gel electrophoresis can be used for determining the molecular weight of proteins?
No! native gels are used to run the proteins in native form, this will tell about the protein's mulimeric nature (ie.monomer or dimer or tetramer etc..).
What is sds-page used for?
SDS - PAGE is apparently used to seperate proteins. The proteins are by nature different sizes. SDS works as a stabilizer by separating proteins according to similar forms.
What is the molecular weight for Vitamin E?
Molecular formula: C29H50O2 Molecular weight: 430.71 CAS Number: 2074-53-5
What is the molecular weight for insulin?
The human insulin protein is composed of 51 amino acids, and has a molecular mass of 5808 Da. Insulin for other species will be somewhat different.
What has higher molecular weight amino acid or protein?
Proteins have the higher molecular weight. They consist of long chains of amino acids joined together.
What does an IPG strip do?
it separates proteins by their isoelectric point along an pH gradient imobilized by a polyacrilamide gel. it is usually the first dimension of separation before using an SDS-PAGE technique to then separate proteins based on molecular weight (2nd dimension).
How separated bands of human serum in a sds page can be recognised?
it could be recognized using a staining day that used to stain proteins in the sample it will separate several band according to molecular weight
What is the molecular weight of butter?
Butter is a mixture of different compounds and therefore does not have any characteristic molecular weight.
What is a molecule of low molecular weight capable of reacting with identical or different molecules of low molecular weight to form a polymer?
This is a catalyst.
What is protein gel..?
If you meant "protein gel electrophoresis" (considering the image on this page) is a very powerful technique and widely used to separate proteins according to their mass, molecular weight and charge. The support most used for this technique is the polyacrylamide.
What can you infer about large molecular-weight proteins that cannot be transported into the nucleus?
It requires a signal device in order to pass through.
Does a protein solution have a high molarity?
No. The reason is that low molecular weight compounds tend to have high molarity. As an example NaF is 42.5 molecular weight. So 42.5g dissolved in 1 liter of water would only be 4.25% but be 1 molar. Proteins tend to have every high molecular weight. So if a protein was say 1000 molecular weight, a 10% solution would contain 100g but only be 0.1 molar.
What is the molecular weight of chymotrypsin?
It has a molecular weight of 21,600 Daltons
Why native gel electrophoresis can be used for determining the molecular weight of proteins?
No! native gels are used to run the proteins in native form, this will tell about the protein's mulimeric nature (ie.monomer or dimer or tetramer etc..).
How does centrifugation separate different kinds of DNA?
Centrifugal force separates different kinds of DNA based on proportions of amino acid base pairs. The AT base pair has a lower molecular weight than CG. Different types of nucleic acids separated into bands.
What is the molecular weight and molecular formula of potassium humate?
molecular structur C9H8K2O4molecular weight: 258.35
