This an enzyme which helps to break down proteins.
a = k/b when a is inversely proportional to b, where k is a constant.
4(3+k)-7 K=0
We need a formula to work this one out.If k = m, then m - 1000If k = 2m, then m = 500If k = 0.5m, then m = 2000You need to provide us with more information before we can answer this.
It could be, if it is a regular stick arrow then no.
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it depends what "too much" is. a concentration up to 1mg/mL or slightly higher can be still ok. proteinase K should digest only proteins, leaving your DNA intact.
I believe the role of proteinase K in a DNA isolation is just to digest proteins. Proteinase K is a protein digesting enzyme. Digesting proteins is important in a DNA isolation because the proteins included in your DNA before you treat it with proK likely include some DNAses. If you didn't use proK, your DNA would degrade very quickly.
As with most protein enzymes change the temperature or change the pH significantly.
Proteinase K is most often used in extracting DNA from while blood cells (or leukocytes). The first step in DNA isolation is to break open the cell and release the cytoplasmic contents, which includes the nucleus, in which we find DNA. Proteinase K is a protease (an enzyme capable of digesting proteins). It is used to digest the cell surface proteins. When cell surface proteins are digested, the integrity of the cell membrane is compromised leading to cell lysis (or the breaking open of the cell)
No difference. Proteinase is the proper term, but protease has become a widely accepted alternative.
An antiproteinase is an inhibitor of proteinase activity.
In order to prepare 50mM TES buffer, you will need to add in approximately 1000 ml of Proteinase K solution. From there, you will need to separate and stack the gels.
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This an enzyme which helps to break down proteins.
Edward A. Fox has written: 'Isolation and characterization of a proteinase inhibitor II gene from Lycopersicon esculentum' -- subject(s): Enzyme inhibitors, Genetics, Proteinase, Tomatoes
In the nucleus of the affected cells.