If accuracy is not a problem the most simple way to produce 20 ml of a 1:400 dilution is to take 1:400 of 20 ml (i.e. 0.05 ml) of the original solution and add 19.95 ml of liquid.
As pipettes are the most precise close to their maximum uptake volume and micro-pipettes are inherently much less precise than pipettes for larger volumes I'd do a two-step dilution.
1. step: 1.000 ml of original solution with 1 ml pipette + 4 times 4.750 ml with 5 ml pipette
2. step: 1.000 ml of solution from step 1 + 4 times 4.750 ml with 5 ml pipette.
1 ml solute to 19 mls solvent. This gives a total volume of 20 (20 fold)
The second sample is 200/10 = 20 times as large. So its mass will be 20 times as great ie 20*20 grams = 400 grams.
Multiply the two dimensions to get the area. The calculation will give you 80 square feet.
20. No it is not a health hazard as raw milk has 50000 cfu/ml and can be consumed by humans.
add 20 grams of dextrose and 4 grams of potato starch to one litre of water. mix the ingredients with agitation. autoclave for 20 minutes at 121 degrees celsius
Add 2 mL of culture to 20 mL of buffer. 2/20 = 1/10
100
400 ÷ 20 = 20 hours (though it is unlikely that the speed would be constant!)
0.05
you can only use the following glassware 5 10 20 25 and 50ml pipette 50 100 200 or 500ml volumetric flasks
400
20 and -20
20 out of 400, as a percentage = 100*(20/400) = 5%
It would be a 20 by 20 ft square
400 - 20 = 380
The answers are sqrt(400) = -20 or +20The answers are sqrt(400) = -20 or +20The answers are sqrt(400) = -20 or +20The answers are sqrt(400) = -20 or +20
400