Why is MgCl2 a variable in PCR?

Updated: 12/22/2022
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14y ago

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Mg2+ complexes with the single stranded DNA that is to be amplified, and becomes the substrate of DNA polymerase. In other words, it helps in the binding of primer (and the subsequent target DNA) to the template DNA. Different volume of Mg2+ exert different complex-forming capabilities, and thus affects the end product of PCR.

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Q: Why is MgCl2 a variable in PCR?
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Related questions

What is the function of mgcl2 in pcr?

it enhance the reaction

Role of magnesium chloride in PCR?

mgcl2 acts as a cofactor for the DNA polymerase used and is necessary for efficient functioning of the pcr

Role of additives in PCR?

Reactants: (dNTPs, template DNA (to be amplified), primers(bind to DNA to begin elongation of strand), DNA Polymerase (elongate DNA), & MgCl2) in buffer + H2O

What are the different types of polymerase chain reaction techniques?

types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR

What is pcr and types of pcr?

PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.

What is the common name for mgcl2?

MgCl2 is magnesium chloride.

105 g of MgCl2 contains how much mol MgCl2?

MgCl2=95grams per mole so 105g/95g is 1.10 mols of MgCl2

What is the molarity of 80g magnesium chloride dissolved in 250ml of water?

MgCl2 Molarity = moles of solute/Liters of solution ( 250 ml = 0.250 L ) Get moles MgCl2 80 grams MgCl2 (1 mole MgCl2/95.21 grams) = 0.8402 moles MgCl2 Molarity = 0.8402 moles MgCl2/0.250 Liters = 3.4 M MgCl2 ----------------

What is the use of dNTP?

The use of dNTP is PCR and multiplex PCR

PCR stands for?

PCR stands for Polymerase Chain Reaction.

What is the correct way to write the formula for magnesium chloride?

The synthesis equation for MgCl2 is Mg + Cl2 -> MgCl2. The decomposition reaction is MgCl2 - heat-> Mg + Cl2.

Draw an atomic representation for MgCl2 on octet rule?