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Why use the 70 percent ethanol?

Updated: 9/19/2023
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12y ago

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to save the ethanol

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Q: Why use the 70 percent ethanol?
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Is 70 percent ethanol the same with 70 percent ethanol?

Absolutely, recent painstaking scientific research has showed that 70% ethanol is the same as 70% ethanol.


Why you use 70 percent ethanol in laminar air flow chamber?

we can also use 100% ethanol or pure ethanol but as it has been tested that 70% is sufficient to kill all the cells and spores of microbes so use this


How do you dilute from ethanol 96 percent to 70 percent?

There's a dilutionfactor of 70/96=0.729, when you make 1 liter, you have to use 729 milliliters of ethanol, then you to add water till 1 liter total.


Why 70 percent ethanol is used for sterilization instead of 100 percent?

70% ethanol has been shown to have the highest killing ability of microbes. Also, 100% ethanol is toxic to the user


Why is 70 percent ethanol used in rna extraction?

The density of 70% ethanol allows RNA settlement or say sedimentation in the vial.


Why do you use 70 percent alcohol and not 90 percent while microbial culture?

Ethanol creates pores in the cell membrane of the microbial cell by solubilizing the lipoproteins of the membrane. The activity of ethanol determines its concentration. If we 90 percent ethanol, the amount of ethanol molecules available to act upon the cell is more and it attacks the cell membrane from all sides. The dissolved lipoprotein forms a kind of layer around the cell, due to which the alcohol is unable to act further and cause damage to the cell. However, if we use 70 percent ethanol, the amount of ethanol molecules are few and it is not able to attack the cell from all sides. Thus, pores are created intermittently in the plasma membrane, the ethanol penetrates the cell and damages it completely. So, 70 percent ethanol is preferable to 90 percent when using microbial cultures.


Why 70 percent percent ethanol used in labs?

70% is the most effective concentration to denature poteins so killing bacteria. At 95% the ethanol just evaporates and leaves the protein untouched. Below 70% does not denature proteins. It also makes the ethanol less flammable.


How does one prepare 500ml of 70 percent ethanol?

150 ml ethanol to 350 ml distilled water


Why is 70 percent ethanol or any diluted alcohol for that matter better at penetrating cell membranes than undiluted alcohols?

70 percent ethanol means it has 30 percent of water.so due to natural osmosis water can enter thr cell membrane.the ethanol thus moves inside along with water and hence has a beter penetrating power than undiluted ethanol.


What is the total volume of 70 percent ethanol from 1600ml ethanol?

If I understand you correctly, we are trying to find the volume of 70% of 1600 ml of ethanol. That said: here is how you do it:1. Change 70% into a decimal. You do this by moving the decimal point two places to the right. This would turn 70 into .702. Multiply .70 x 1600.3. The answer is: 1120mL.


Why 95 percent ethanol is used in laboratory?

So as not to 'waste' too much ethanol. To use 95 % ethanol allows some ethanol to be saved!


What volume of ethanol 96 percent should be added to 500 ml of 70 percent ethanol such that when the mix is made to 2.5l with water the final conc of ethanol is 55 percent?

First calculate how much ethanol you need in the final solution. 55% X 2.5l = 1.375l Then calculate how much ethanol you already have in the solution. 0.5l X 70% = 0.35l Now take the difference between those two numbers as the extra amount needed. 1.375l - 0.35l = 1.025l Now correct for the fact that you are adding only 96% ethanol. 1.025l / 96% = 1.068l Answer: 1.068l