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How do you calculate total phenolics by folin ciocalteu method?
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How do you prounced folin-ciocalteu?
The word "folin-ciocalteu" refers to the scientific phenol reagent named after the two scientists, Folin and Ciocalteu. These names are of Swedish and Romanian origin. The combination is pronounced similar to "Faux-lean-cho-cal-the-oh."
What is the reaction mechanism of the folin ciocalteu reagent and poly phenols?
The Folin-Ciocalteu reagent which is a mixture of tungstates and molybdates works on the mechanism of oxidation-reduction reaction. The method strongly relies on the reduction of the mixture heteropolyphosphotungsates-molybdates by the phenolic compound which results in the formation of blue coloure chromogen. The phenolic compounds react with Folin-Ciocalteu reagent only under basic conditions adjusted by sodium carbonate solution. Under Basic conditions it has been observed that the phenolic compound undergoes dissociation to form a phenolate anion which reduces the Folin-Ciocalteu reagent i.e. the mixture of tungstates and molybdates rendering a blue coloured solution. The colour intensity of the formed blue chromogen can be measured by the absorbance readings using a spectrophotometer
How folin ciocalteu works in total phenolic content method?
Folin's method measures -OH groups in a sample based on the fact that light absoption increases as OH groups in a sample increases. So, this method do not measure the exact amount of polyphenols because polyphenols are not the only compound has OH group if other OH group containing compounds are in the same medium such as protein, carbohyrate, vitamin C, and saponin.
How folin-ciocalteu works in total phenolic content method?
Folin's method measures -OH groups in a sample based on the fact that light absoption increases as OH groups in a sample increases. So, this method do not measure the exact amount of polyphenols because polyphenols are not the only compound has OH group if other OH group containing compounds are in the same medium such as protein, carbohyrate, vitamin C, and saponin.
What are the reagents used in the estimation of antioxidants?
There are various reagents that are used in the estimation of antioxidants. Folin-Ciocalteu and DPPH are the most common reagents used in determining antioxidant activity.?æ
Why protein estimation is done at 660 nanometer in folin lowry method?
because folin lowry reagent has absorption maxima at 660 nm
How to make in laboratory Folin and Wu's solution?
A description of the method and reagents is at this link.
When did Otto Folin die?
Otto Folin died on 1934-10-23.
When was Otto Folin born?
Otto Folin was born on 1867-04-04.
When did Léopold de Folin die?
Léopold de Folin died in 1896.
When was Léopold de Folin born?
Léopold de Folin was born in 1817.
When was Denis Folin born?
Denis Folin was born on 1989-05-13.
