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If PKa value is less than 0?

Updated: 9/24/2023
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Q: If PKa value is less than 0?
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we can't tell the charge of lysine at 4.5 because the PKa is not given. we can only know what charge it carries with the PKa value on carboxyl group or amine group.And to know the charge remember when PH is less than PKa it is always 0 while when PH is higher than PKa it is always -1 on carboxyl group. For the amine group it is always +1 as the PKa is always higher than the PH. so for example we have PKa1 2.19 PKa 5.2 for caboxyl group and 9.67 For amino group. to deter mine the charge is on pka1 the charge -1 because PH4.5 is higher than the pka,while on pka 2 the charge is 0 as PH 4.5 is lower than PKa.while for amine group it is + 1 because PKa is higher than PH4.5 so there the charge on lysine will be calculated -1+0+1=0. that's how i understand it


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