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Q: Is Methylene blue positive or negative in dye solution?
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Will staph grow on EMB agar?

No, it will not. Methylene blue agar is used to identify gram negative bacteria, staphylococcus is gram positive.

What is the function of methylene blue solution on polluted water samples?

Polluted water sample will decolorize the methylene blue solution faster than a pure water sample.

Do EMB plate dyes inhibit growth of Gram-positive or negative bac?

The dyes (eosin Y and methylene blue) inhibit the growth of gram- positive bacteria.

Why do gram positive bacteria retain the primary stain methylene blue while gram negative bacteria are decolorized by rinsing with ethanol?


Is it possible to substitute methylene blue for nigrosin in the negative stain?


What are the EMB test results for Staphylococcus epidermidis?

The Eosine Methylene Blue test is selective for only gram-negative bacteria. It is possible for gram-positive bacteria to grow, but it would barely grow. Staphylococcus are gram-positive spherical bacteria. So it would not grow on the Eosine methylene Blue Agar.

How do you make sugar crystals that are blue?

Adding methylene blue to sugar water solution.

Why is methylene blue is an appropriate dye for staining bacterial cells?

It is used in gram staining to differentiate gram negative and gram positive bacteria. After being dyed, the cells are washed with ethanol. Gram positive bacteria will retain the methylene blue due to the amount of peptidoglycan in their cell walls, where gram negative cells will not. Iodine is used as a counter stain, which is up-taken by gram negative cells. After the gram staining procedure is finished, gram positive cells will appear dark purple or blue due to the retained methylene blue. Gram negative cells will appear pink or red due to the iodine counter stain.

Why methylene blue inhibits gram positive bacteria?

because it is acidic?

Would methylene blue stain be substituted for safranin as the counter stain in this procedure?

Methylene blue is a stain used in microbiology. The dye attaches to the object, which allows what would normally be fairly see-through to be visible to the microscope.

What is the substances in a living cell that has same function as methylene blue?


Why are tomato cells stained with methylene blue as opposed to iodine solution?

do not know