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Q: Is Methylene blue positive or negative in dye solution?
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What is the function of methylene blue solution on polluted water samples?

Polluted water sample will decolorize the methylene blue solution faster than a pure water sample.


What ingredients of EMB agar inhibit the growth of gram positive organisms?

Eosin and methylene blue dyes present in EMB (Eosin Methylene Blue) agar inhibit the growth of gram-positive organisms by interfering with their ability to take up nutrients and causing damage to their cell walls. This makes the medium selective for gram-negative bacteria, allowing them to grow while inhibiting the growth of gram-positive bacteria.


Will staph grow on EMB agar?

Staphylococcus bacteria typically do not grow well on Eosin Methylene Blue (EMB) agar. EMB agar is selective for gram-negative bacteria, particularly Enterobacteriaceae, and contains dyes that inhibit the growth of gram-positive bacteria like Staphylococcus.


What are the EMB test results for Staphylococcus epidermidis?

The Eosine Methylene Blue test is selective for only gram-negative bacteria. It is possible for gram-positive bacteria to grow, but it would barely grow. Staphylococcus are gram-positive spherical bacteria. So it would not grow on the Eosine methylene Blue Agar.


Is it possible to substitute methylene blue for nigrosin in the negative stain?

no


How do you make sugar crystals that are blue?

Adding methylene blue to sugar water solution.


What is the function on bile in Eosin Methylene Blue agar?

Bile salts in Eosin Methylene Blue agar inhibit the growth of Gram-positive bacteria, allowing for the selective growth of Gram-negative bacteria. This helps in the detection and differentiation of organisms based on their ability to ferment lactose and produce acid.


What ingridient makes EMB to be selective?

Eosin methylene blue (EMB) agar is selective due to the presence of dyes eosin Y and methylene blue, which inhibit the growth of Gram-positive bacteria. This allows for the selective isolation of Gram-negative bacteria, particularly coliforms.


Would methylene blue stain be substituted for safranin as the counter stain in this procedure?

Methylene blue is a stain used in microbiology. The dye attaches to the object, which allows what would normally be fairly see-through to be visible to the microscope.


What is the substances in a living cell that has same function as methylene blue?

NADH


How do you make methylene blue solution?

To make a methylene blue solution, you can dissolve methylene blue powder in water at a desired concentration. The typical concentrations range from 0.1% to 1%. Make sure to use proper safety precautions, such as wearing gloves and protective eyewear, as methylene blue can stain skin and clothing.


Why is methylene blue is an appropriate dye for staining bacterial cells?

Methylene blue is an appropriate dye because it readily binds to the negatively charged components of bacterial cells, such as nucleic acids and proteins. This binding allows for clear visualization of bacterial morphology and intracellular structures under a microscope. Additionally, methylene blue is a relatively simple and cost-effective stain to use in microbiology.