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Methylene blue is a cationic dye, meaning it has a positive charge in solution. This positive charge allows it to bind effectively to negatively charged components, such as nucleic acids and proteins, making it useful in various biological and staining applications. In acidic or neutral pH conditions, methylene blue remains positively charged, contributing to its effectiveness as a dye.
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Principle of May-Grünwald stain?
The May-Grünwald stain is a vital staining technique used primarily in hematology to visualize blood cells and their components. It relies on a combination of eosin and methylene blue, which selectively stain the cytoplasmic components and nuclei of cells, respectively. The eosin imparts a pink color to eosinophilic granules and cytoplasm, while methylene blue stains the nuclei blue, allowing for differentiation of various cell types. This staining method is particularly useful for identifying abnormalities in blood smears.
What does a really faint blue negative line mean on an ept?
your pregnant!!!!!!!!!!!!!!!!!!!!!!!
What color is the negative and which one is the negative on my rear right speaker. ( ones blue and ones teal) Chevy Impala 2010?
In a typical wiring setup for speakers, the negative terminal is usually marked or coded. For your 2010 Chevy Impala, if one wire is blue and the other is teal, the negative wire is likely the blue one. However, it's always best to double-check with a multimeter or refer to your vehicle's manual for confirmation.
Why All solutions are colourless true or false?
False. Not all solutions are colorless; the color of a solution depends on the solute dissolved in the solvent. For example, a solution of copper sulfate is blue, while a solution of potassium permanganate is purple. The presence of certain ions or compounds can impart color to a solution, making it visible to the eye.
Bag contains 5 blue blocks and 3 red blocks and 2 yellow blocks so what is the probability that someone will draw a red block and replace it and then a draw a blue block at random?
The probability of drawing a particular color is the number of blocks that color, over the total number of blocks. Since these are disjoint events, the solution is the product of the two probabilities of the draws. So: P(red, blue) = P(red) * P(blue) = (3/10) * (5/10) = 15/100 or 3/20
What is the function of methylene blue solution on polluted water samples?
Polluted water sample will decolorize the methylene blue solution faster than a pure water sample.
What ingredients of EMB agar inhibit the growth of gram positive organisms?
Eosin and methylene blue dyes present in EMB (Eosin Methylene Blue) agar inhibit the growth of gram-positive organisms by interfering with their ability to take up nutrients and causing damage to their cell walls. This makes the medium selective for gram-negative bacteria, allowing them to grow while inhibiting the growth of gram-positive bacteria.
Will staph grow on EMB agar?
Staphylococcus bacteria typically do not grow well on Eosin Methylene Blue (EMB) agar. EMB agar is selective for gram-negative bacteria, particularly Enterobacteriaceae, and contains dyes that inhibit the growth of gram-positive bacteria like Staphylococcus.
Is it possible to substitute methylene blue for nigrosin in the negative stain?
no
What are the EMB test results for Staphylococcus epidermidis?
The Eosine Methylene Blue test is selective for only gram-negative bacteria. It is possible for gram-positive bacteria to grow, but it would barely grow. Staphylococcus are gram-positive spherical bacteria. So it would not grow on the Eosine methylene Blue Agar.
How do you make sugar crystals that are blue?
Adding methylene blue to sugar water solution.
What is the behavior of methylene blue towards the animal charcoal?
i think the methylene blue will be make aqua blue because the charcoal will penerate the color of methylene blue,,,there are absorption process,,,in the charoal between the methylene blue.... (kharlz)
What is the function on bile in Eosin Methylene Blue agar?
Bile salts in Eosin Methylene Blue agar inhibit the growth of Gram-positive bacteria, allowing for the selective growth of Gram-negative bacteria. This helps in the detection and differentiation of organisms based on their ability to ferment lactose and produce acid.
What ingridient makes EMB to be selective?
Eosin methylene blue (EMB) agar is selective due to the presence of dyes eosin Y and methylene blue, which inhibit the growth of Gram-positive bacteria. This allows for the selective isolation of Gram-negative bacteria, particularly coliforms.
Would methylene blue stain be substituted for safranin as the counter stain in this procedure?
Methylene blue is a stain used in microbiology. The dye attaches to the object, which allows what would normally be fairly see-through to be visible to the microscope.
What is the substances in a living cell that has same function as methylene blue?
NADH
can you see methylene blue as your counterstaining the gram stain proceure?
Yes, methylene blue can be used as a counterstain in the Gram staining procedure, although it is not the most common choice. Typically, safranin is used for this purpose, staining Gram-negative bacteria pink. However, methylene blue can provide a contrasting color, allowing for clearer differentiation between Gram-positive (purple) and Gram-negative (blue) bacteria. This alternative can be particularly useful in certain specific applications or educational settings.
