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What is the function of methylene blue solution on polluted water samples?

Polluted water sample will decolorize the methylene blue solution faster than a pure water sample.


What ingredients of EMB agar inhibit the growth of gram positive organisms?

Eosin and methylene blue dyes present in EMB (Eosin Methylene Blue) agar inhibit the growth of gram-positive organisms by interfering with their ability to take up nutrients and causing damage to their cell walls. This makes the medium selective for gram-negative bacteria, allowing them to grow while inhibiting the growth of gram-positive bacteria.


Will staph grow on EMB agar?

Staphylococcus bacteria typically do not grow well on Eosin Methylene Blue (EMB) agar. EMB agar is selective for gram-negative bacteria, particularly Enterobacteriaceae, and contains dyes that inhibit the growth of gram-positive bacteria like Staphylococcus.


Is it possible to substitute methylene blue for nigrosin in the negative stain?

no


What are the EMB test results for Staphylococcus epidermidis?

The Eosine Methylene Blue test is selective for only gram-negative bacteria. It is possible for gram-positive bacteria to grow, but it would barely grow. Staphylococcus are gram-positive spherical bacteria. So it would not grow on the Eosine methylene Blue Agar.


How do you make sugar crystals that are blue?

Adding methylene blue to sugar water solution.


What is the behavior of methylene blue towards the animal charcoal?

Methylene blue adsorbs onto the surface of animal charcoal through hydrophobic interactions and van der Waals forces. This adsorption process removes methylene blue molecules from solution, effectively purifying or decolorizing it.


What is the function on bile in Eosin Methylene Blue agar?

Bile salts in Eosin Methylene Blue agar inhibit the growth of Gram-positive bacteria, allowing for the selective growth of Gram-negative bacteria. This helps in the detection and differentiation of organisms based on their ability to ferment lactose and produce acid.


What ingridient makes EMB to be selective?

Eosin methylene blue (EMB) agar is selective due to the presence of dyes eosin Y and methylene blue, which inhibit the growth of Gram-positive bacteria. This allows for the selective isolation of Gram-negative bacteria, particularly coliforms.


What is the substances in a living cell that has same function as methylene blue?

NADH


Would methylene blue stain be substituted for safranin as the counter stain in this procedure?

Methylene blue is a stain used in microbiology. The dye attaches to the object, which allows what would normally be fairly see-through to be visible to the microscope.


How do you make methylene blue solution?

you can get methylene blue powder from a scientific store, it comes in powdered form. its pretty soluble in water and alcohol etc. the stain is made by dissolving an appropriate amount on methylene blue in a solvent, e.g for 0.1 dissolve 0.1% gram of methylene blue in 100 gram water, for 9% dissolve 9 grams