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Certainly rt-PCR is qualitative and can also theoretically be quantitative. Anneal the RNA to get a 1:1 RNA to DNA copy, then proceed with quantitative PCR.
In qualitative PCR specific DNA fragment is detected while in quantitative PCR our target DNA sequence not only is detected but its amount is determined (after reaction we can calculate the amount of DNA we had in our sample)
: Differentiate between quantitative and real time PCR.
Quantitative PCR Technology is used in biochemistry, in particular molecular biology. The PCR stands for polymerase chain reaction and is used to "amplify" pieces of DNA to make millions of copies of a particular DNA strand.
There are several advantages of using real-time PCR over other methods. Real-time PCR assays are thousand folds more sensitive than RNase protection assays or dot blot hybridization. It allows you to quite precisely calculate and compare of the amount of template in each cycle, instead of determining the amount of product at the end of the reaction. Quantitative RT-PCR is commonly used for clinical applications. For example, you could use this method to quantify the amount of HIV RNA particles per ml of blood plasma in a patient who is undergoing treatment with antiviral drugs to see if it's working or not. The main problem with real-time PCR is that it requires specialised thermal cyclers (PCR machine) with fluorescence monitors and its reagents are quite expensive.
my HBV VIRAL LOAD BY REAL TIME PCR IS 165 MEANS
PCR allows amplification of DNA for a specific gene, after too many cycles of PCR the result will reach saturation, basically meaning all of the DNA has been amplified. Conventional PCR will basically tell you whether or not a gene is expressed in your sample. This can be done semi-quantitavely if the PCR is performed for a low number of cycles, ie it will tell you whether one sample expresses more of your gene of interest than another sample. The results are seen by separating the PCR products by agarose gel/ethidium bromide electrophoresis. Real-time PCR will record exactly what cycle of PCR a detectable level of amplified product became detectable, giving a far more accurately quantifiable estimation of gene expression.
TaqMan Gene Expression Assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a FAM or VIC dye label on the 5' end, and minor groove binder (MGB) nonfluorescent quencher (NFQ) on the 3' end.
RT PCR Test is the most accurate test while pcr test or rapid test can get you results very quickly, the results may not always be accurate.
TB PCR blood test is done for rapid detection of Tuberculosis. This has proven to be the fastest and most effective detection method.
With ELISA test or other allergen test like pcr or atp.
The basic principles of quantitative real time PCR include having the ability to target a particular DNA or RNA sample in a sample relative to either a standard or another sample that has been subjected to treatment.