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How many colors make the color Violet?
Violet is a secondary color. Secondary colors are achieved by mixing two of the three primary colors in equal proportions. Violet is made by mixing Blue and Red, therefore the answer is two.
What is the definition of analogous colors?
Colors that are related to each other are analogous. They are neighbors on the color wheel and share a common color. For example, blue violet, violet, and red violet. they all contain red. The Violet (a secondary color) has been made by mixing red and blue (both primary colors). The blue-violet and red-violet (both tertiary colors) have been made by mixing the secondary color (Violet) with the primary color included in it's name. They are analogous, or relatives, because they all contain red. This holds true for any three colors on the color wheel which directly in contact with each other. See the related link for more on mixing colors.
What is the genre of the song violet hour by sea wolf?
"Violet Hour" by Sea Wolf is considered to be an alternative-rock song according to iTunes.
How do you prepare 1 percent gentian violet?
1) take 10gm of gentian violet. then add to them: 2) Alcohol 70% ( H2O).... to 1000 ml
What is analogous colors that is on the color wheel?
An analogous scheme in association with the use of the Colour wheel is the usage of adjacent colours. EG: Red, Red-violet and Violet.
What is crystal violet iodine complex?
In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .
Explain why crystal violet would not be useful in the negative stain technique?
Negative staining techniques are designed to stain everything BUT the bacterial cells. This allows us to see the cells unstained and helps us observe their morphology (how the cells are shaped and how they group together). The cells have an overall negative charge on their surface, so they naturally attract positive charges. Crystal Violet carries a positive charge, so it would adhere to the surfaces of the cells thus staining the cells (which is not what you want in a negative stain!)
What is positive staining?
cells which take up crystal violet stain and retain them because of smal pore size
Why gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is Gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is Iodine used in Gram staining?
Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.
Function of Gram's iodine?
Iodine is used as a mordant in the gram staining procedure to make large crystals when it is used with crystal violet dye. In gram positive cell walls those crystals get stuck and wont get washed off with the alcohol. In gram negative cell walls the crystals are washed out.
What are the reagents and steps min a Gram stain?
The steps in Gram staining are:1. crystal violet added to the smear2. iodine, the mordant (this fixes the violet)3. a decolorizer made of acetone and alcohol4. safranin, the counterstainIf the cell is Gram +, the decolorizer can not remove the violet. If it is Gram -, the decolorizer can remove the violet and the cell can be then colored with the dye, safranin.Bacteria are grouped in 4 groups by Gram stain:Gram-positive, the cell wall retains crystal Violet.Gram-negative, the cell wall does not retain crystal Violet.Graham not reactive, no staining whatsoever.Graham variable, uneven staining.
What is gram positive strain?
Gram-positive bacterium are those that are stained dark blue or violet by Gram Staining. This is in contrast to Gram-Negative Bacterium, which cannot retain the crystal violet stain, instead taking up the counter-stain and appearing red or pink. Gram-positive organisms are able to retain the crystal violet stain because of the high amount of peptidoglycan in the cell wall. Gram-positive cell walls typically lack the outer membrane found in Gram-negative bacteria.
Function of crystal violet in gram stain?
Well if you stained with only crystal violet, then they would all be violet! If you do a Gram Stain the right way, you end up getting Gram positive being violet or purple and Gram Negative being red or pink.
Why gram staining is classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
How can you remove crystal violet in a Gram Stain?
You won't be able to positively distinguish gram negative from gram positive organisms. Crystal violet is used first so that gram positive organisms will take up the dye and this color is not affected by safrinin later on. Gram negative organisms will lose the purple coloring during the decolorizer step, therefore need to be counterstained so that you can view the organisms.
