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What is the function of crystal violet in the gram staining process?

Crystal violet is used in the Gram staining process to initially stain all bacteria purple. This helps differentiate between Gram-positive and Gram-negative bacteria based on their ability to retain or lose the crystal violet dye during the staining process.


Is the crystal violet test result positive or negative?

The crystal violet test result is positive.


Is anthrax gram positive or negative?

Anthrax is a gram-positive bacterium, meaning it retains the crystal violet stain in the Gram staining procedure.


What is the purpose of crystal violet in the process of gram staining?

Crystal violet is used in the process of gram staining to initially stain all bacteria purple. This helps differentiate between gram-positive and gram-negative bacteria based on their cell wall composition.


What is the role of crystal violet in the process of gram staining?

Crystal violet is a purple dye used in the gram staining process to initially stain all bacteria cells. It helps differentiate between gram-positive and gram-negative bacteria based on their cell wall composition.


Why is Iodine used in Gram staining?

Iodine is used in Gram staining as a mordant, which helps to bind the crystal violet dye to the cell wall of bacteria. This mordant-iodine complex forms larger complexes with the crystal violet dye, making it difficult for the dye to be washed away during the decolorization step. This allows for differentiation of Gram-positive and Gram-negative bacteria based on their ability to retain the crystal violet dye.


Explain why crystal violet would not be useful in the negative stain technique?

Negative staining techniques are designed to stain everything BUT the bacterial cells. This allows us to see the cells unstained and helps us observe their morphology (how the cells are shaped and how they group together). The cells have an overall negative charge on their surface, so they naturally attract positive charges. Crystal Violet carries a positive charge, so it would adhere to the surfaces of the cells thus staining the cells (which is not what you want in a negative stain!)


Function of Gram's iodine?

Iodine is used as a mordant in the gram staining procedure to make large crystals when it is used with crystal violet dye. In gram positive cell walls those crystals get stuck and wont get washed off with the alcohol. In gram negative cell walls the crystals are washed out.


What are the functionally analogous pair of stains?

Crystal violet and safranin are functionally analogous pair of stains in the Gram staining technique. Crystal violet stains gram-positive bacteria purple/blue, while safranin counterstains gram-negative bacteria pink/red.


Why is timing so critical when decolourizing in Gram staining?

Timing is critical when decolorizing in Gram staining because if the decolorizer is left on for too long, it can wash away the crystal violet stain from Gram-positive cells, leading to a false negative result. Conversely, if the decolorizer is not left on long enough, the crystal violet stain may not be fully removed from Gram-negative cells, leading to a false positive result. Timing ensures accurate differentiation between Gram-positive and Gram-negative bacteria.


What is positive staining?

cells which take up crystal violet stain and retain them because of smal pore size


What is crystal violet iodine complex?

Crystal violet iodine complex is a chemical compound formed when crystal violet dye interacts with iodine. This complex is commonly used in microbiology as a stain for bacterial cells, allowing them to be visualized under a microscope. The crystal violet iodine complex binds to the peptidoglycan layer of bacterial cell walls, imparting a purple color to the cells.