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Q: Is crystal violet positive or negative staining?
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Is anthrax gram positive or negative?

Anthrax is a gram-positive bacterium, meaning it retains the crystal violet stain in the Gram staining procedure.


What is crystal violet iodine complex?

Crystal violet iodine complex, also known as the Gram's iodine, is used in the Gram staining technique to differentiate between Gram-positive and Gram-negative bacteria. It works by forming a complex with the peptidoglycan layer of the bacterial cell wall, allowing for visualization under a microscope.


Why is Iodine used in Gram staining?

Iodine is used in Gram staining as a mordant, which helps to bind the crystal violet dye to the cell wall of bacteria. This mordant-iodine complex forms larger complexes with the crystal violet dye, making it difficult for the dye to be washed away during the decolorization step. This allows for differentiation of Gram-positive and Gram-negative bacteria based on their ability to retain the crystal violet dye.


Explain why crystal violet would not be useful in the negative stain technique?

Crystal violet is a basic dye, meaning it has a positive charge and stains negatively charged structures well. In the negative stain technique, the background is stained, leaving the specimen colorless. Using crystal violet would inadvertently stain the specimen, resulting in a false positive.


Function of Gram's iodine?

Gram's iodine is used in the Gram staining method to differentiate bacteria into two major groups: Gram-positive and Gram-negative. It functions by forming complexes with the crystal violet dye in the cell wall of the bacteria. This allows for the retention of the dye by Gram-positive bacteria, while it is washed off from Gram-negative bacteria.


What are the functionally analogous pair of stains?

Crystal violet and safranin are functionally analogous pair of stains in the Gram staining technique. Crystal violet stains gram-positive bacteria purple/blue, while safranin counterstains gram-negative bacteria pink/red.


Why is timing so critical when decolourizing in Gram staining?

Timing is critical when decolorizing in Gram staining because if the decolorizer is left on for too long, it can wash away the crystal violet stain from Gram-positive cells, leading to a false negative result. Conversely, if the decolorizer is not left on long enough, the crystal violet stain may not be fully removed from Gram-negative cells, leading to a false positive result. Timing ensures accurate differentiation between Gram-positive and Gram-negative bacteria.


What is positive staining?

cells which take up crystal violet stain and retain them because of smal pore size


Why gram staining classified as differential staining?

Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining


Why is Gram staining classified as differential staining?

Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining


What color will gram stain positive turn into?

Gram-positive bacteria will retain the crystal violet dye during the gram staining process, appearing purple/blue under a microscope.


Is iodine a basic stain?

No, iodine is not a basic stain. Iodine is commonly used in Gram staining to identify bacteria as either Gram-positive or Gram-negative based on their cell wall composition. It acts as a mordant in the staining process and helps to fix the crystal violet stain in Gram staining.