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What are the advantages of using A readings rather than percent T as a means of estimating microbial growth?
Tibourik
The density of a cell suspension is expressed as absorbance (A) rather than percent T, since A is directly proportional to the concentration of suspended cells, whereas percent T is inversely proportional to the concentration of suspended cells. Therefore, as the turbidity of a culture increases, the A increases and percent T decreases, indicating growth of the cell population in the culture.
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