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The two most important sequences are the expression cassette and the selection marker.
The expression cassette contains a promoter and termination sequence, with a multiple cloning site sandwiched in between. It is the section of the plasmid that integrates into the genome of the target organism. The gene of interest is cloned into the multiple cloning site by digesting both plasmid and gene fragment with restriction enzymes that produce complementary ends. Once the plasmid has been transferred into the target organism the promoter sequence determines when the gene is transcribed. Some promoters are always active (Cauliflower Mosaic Virus 35S is most common) and others are developmentally or environmentally regulated, so that the gene is only expressed at specific times. The termination sequence determines where transcription stops.
The selection marker is used to determine which organisms are carrying the gene of interest. It usually contains a promoter, resistance gene and termination sequence and forms part of the expression cassette. In most cases antibiotic resistance is used as selection. For example the nptII gene encodes for kanamycin resistance.
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This is an artificial genetic sequence from combining two other sequences in a plasmid?
Recombinant DNA
What is an altered plasmid?
I think that an altered plasmid is one that has just undergone transformation, so it has the two combined vectors inside of it.
Give two reasons why a plasmid is useful for DNA transfer?
A plasmid is relatively small in length and can be manipulated to have different genes on it.
What are the six different types of vectors?
Plasmid and Viral are two
When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
This is an artificial genetic sequence from combining two other sequences in a plasmid?
Recombinant DNA
Give two reason why plasmid is useful for DNA transfer?
A plasmid is relatively small in length and can be manipulated to have different genes on it.
What is an altered plasmid?
I think that an altered plasmid is one that has just undergone transformation, so it has the two combined vectors inside of it.
Give two reasons why a plasmid is useful for DNA transfer?
A plasmid is relatively small in length and can be manipulated to have different genes on it.
What are the six different types of vectors?
Plasmid and Viral are two
When is a plasmid considered a recombinant plasmid?
When the original function of the gene in the plasmid is altered or another gene is inserted in the non- coding region of the plasmid is called the recombinant plasmid.
Why was it important to find an enzyme that would cut the plasmid at only one site?
If the plasmid were cut at the replication, it would not be able to reproduce & transfer genetic information to its host cell.
If you took a linear piece of DNA and cut it with the restriction enzyme EcoRI and it had three restriction sites for EcoRI, how many fragments would you produce What if you had a circular piece of DNA?
If the plasmid has 3 recognition sequences for a given restriction endonuclease, then 4 linear DNA fragments are obtained because, if the DNA is linear then the number of fragments obtained is (N+1) whereas if the DNA is circular then the number of fragments obtained will be N for N recognition sequences for the given restriction endonuclease in a plasmid.
What is the function of the Ti plasmid?
what is the function of the plasmid
The restriction enzyme used in constructing hybrid molecules of certain gene sequences and plasmid DNA acts by?
opening DNA molecules at specific sites,leaving sticky ends exposed. find me on kik one1992
Quantifier sequences Do the following two statements mean the same thing?
To determine if the following two statements mean the same thing, you would need to offer the quantifier sequences. Then, you could compare the sequences to determine if they are the same.
Which is the plasmid that increases resistance to antibiotics?
R-plasmid
