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Incorrect measurement of the incident intensity, Io. If the measurement if Io is less than the transmitted intensity, I, then the absorbance read-out (calculated as log(Io/I) will be negative.

This error usually results from unmatched or misaligned cells, or in an improper blank solution (used to measure the Io signal).

Tom O'Haver

http://terpconnect.umd.edu/~toh/

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