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Incorrect measurement of the incident intensity, Io. If the measurement if Io is less than the transmitted intensity, I, then the absorbance read-out (calculated as log(Io/I) will be negative.
This error usually results from unmatched or misaligned cells, or in an improper blank solution (used to measure the Io signal).
Tom O'Haver
http://terpconnect.umd.edu/~toh/
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What causes your car to accelerate and go out of control?
too many beers dude ..or the accelerator may be getting stuck
How many grams of fat in coors beer?
All beers are fat free, it's the sugar content that causes weight gain.
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