Reagent Blank : Take reagent and add deionised water (in place of sample to be tested). Now measure the OD at specific wavelength --> this OD is your reagent blank. Substract this OD from your test result (with sample) to avoid any false +ve effect due to colour of reagents itself.Sample Blank : Take sample and measure the OD without adding reagents --> this OD is your sample blank. Substract this OD from your test result to avoid any false +ve effect due to colour and turbidity of sample itself. As it is the fact that colour and turbidity of each sample would vary from one to another.So now it is clear that Reagent blank is used to avoid bias due to colour of reagents and Sample blank is used to avoid bias due to sample itself.
a sample is a sample sized piece given... a sample size is the amount given in one sample
An experimental sample is an experiment that is just a sample of what you are looking for.
The sample size can be any number that you choose based on a number of criteria.Factors to consider include:How large a sample you can afford. Sampling does not come free, nor does data cleaning, data processing etc.How quickly you need an answer.How variable you expect the answer to be (you could be wrong, but a rough idea will help decide on the sample size).Whether or not the characteristic that you want to look at is rare. For example, you'd need a bigger sample to find even one occurance of a household in which someone had some rare allergy.The consequences of getting the answer wrong. Why spend huge amounts of money to find out what colour cars they own.
Benedicts solution changes through a range of colours (blue, green, orange, red) according to how much reducing sugar is present in the sample. This can be used to give a rough answer to the question "How much sugar is in the sample?", but is not accurate enough (because of the blending between one colour and the next), to be called a quantitative test.
aPTT
Therapeutic range for aPTT is 45-75 seconds.
Because a plastic bottle can contaminate the sample; and a glass bottle is fragile.
7.48
Silver chloride is light sensitive. Light photons break the salt down into silver metal and chlorine gas. Using a dark bottle protects the salt from the light that would degrade the sample.
This is to preserve the urine sample until its tested.
aptt is 100 seconds
0.84-1.22
greenred
Bottle green is bottle green.That is the actual name for the colour. It's a dark shade of green.The first time the colour was used is in 1816 in England for beer bottles, hence the name, bottle green.The colour is also being used on the Flag of Bangladesh and the police uniforms in Northern Ireland.
hypercoagulatize state
shaking the sample before transferring it into a sterile universal bottle is an important step in many microbiological experiments as it helps to ensure that the sample is well-mixed and that any microorganisms present are evenly distributed throughout the sample.