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What is the difference between reagent blank and sample blank?
Wiki User
∙ 10y ago
Reagent Blank : Take reagent and add deionised water (in place of sample to be tested). Now measure the OD at specific wavelength --> this OD is your reagent blank. Substract this OD from your test result (with sample) to avoid any false +ve effect due to colour of reagents itself.
Sample Blank : Take sample and measure the OD without adding reagents --> this OD is your sample blank. Substract this OD from your test result to avoid any false +ve effect due to colour and turbidity of sample itself. As it is the fact that colour and turbidity of each sample would vary from one to another.
So now it is clear that Reagent blank is used to avoid bias due to colour of reagents and Sample blank is used to avoid bias due to sample itself.
Wiki User
∙ 10y ago
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If you want to read the absorbance at 595nm of a protein solution mixed with the colorizing solution called Bradford reagent what is a suitable reagent blank?
A suitable reagent blank for measuring the absorbance of a protein solution mixed with Bradford reagent at 595nm would be a blank containing all components of the reaction except the protein sample, such as water or buffer mixed with the Bradford reagent. This blank will account for any background absorbance contributed by the reagent itself, allowing for a more accurate measurement of the protein concentration.
What is a reagent blank?
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Why do we add water to reagent blank?
We add water to the reagent blank to ensure that any impurities or contaminants in the water or the other reagents used will be accounted for in subsequent measurements, allowing for a more accurate determination of the analyte concentration. This helps to correct for any background signal or interference that may affect the final results.
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Fill in the blank. The measure of an angle is the difference between the directions of its two .?
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Fill in the blank The measure of an angle is the difference between the directions of its two?
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What is the purpose of blank samples?
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Why do you use a reagent blank and not just distilled water to zero the spectrophotometer?
I know exactly what this question is from. This is an experiment testing the amount of dissolved iron in a solution, correct? If so, then this is the answer I came up with: The solutions being tested are not simply iron dissolved in water; they contain other substances, like sulfuric acid, sodium citrate, etc. These other materials will affect the absorption of the sample solutions, so they must be zeroed out. Using only distilled water does not effectively account for this, so a reagent blank, having been treated in the same way as all of the other solutions, must be used instead. This answer was not confirmed by my professor, but it is what I came up with, and it sounds reasonable to me. Hope that helps!
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