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if you are doing isocratic elution nothing will change at all but in case pf gradient analysis elution order may change.

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โˆ™ 2013-01-05 20:46:37
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Q: What happen you changed the percentage composition of mobile phase in HP LC?
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Bad things happen. The mobile phase evaporates and thus it's composition changes. The vapor phase above the chromatogram is not saturated or at equilibrium, and this affects the movement of the sample on the solid phase.

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