if you are doing isocratic elution nothing will change at all but in case pf gradient analysis elution order may change.
In isocratic HPLC, the mobile phase composition remains constant throughout the entire run, leading to constant elution times for all analytes. In gradient HPLC, the mobile phase composition is changed during the run, allowing for better separation of complex mixtures by adjusting the solvent strength over time.
99.9
Mobile Forces happened in 2002.
Mobile Golf happened in 2001.
DJMax Mobile happened in 2009.
Rad Mobile happened in 1990.
Operation Mobile happened in 2011-11.
95%
Bad things happen. The mobile phase evaporates and thus it's composition changes. The vapor phase above the chromatogram is not saturated or at equilibrium, and this affects the movement of the sample on the solid phase.
Duke Nukem Mobile happened in 2004.
Wild Arms Mobile happened in 2006.
Empire Earth Mobile happened in 2005.