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Q: What is an internal standard and why is it used?
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Why internal standard you used in hplc method?

Internal standard can be used for calibration by plotting the ratio of the analyte signal to the internal standard signal as a function of the analyte concentration of the standards. This is done to correct for the loss of analyte during sample preparation or sample inlet.


Why is o-xylene used as an internal standard in this experiment?

Oxylene is used as an internal standard in experiments because it absorbs a wide area. It also absorbs other compounds easily.


What is the characteristics of an internal standard?

Internal Standard(IS) is similar in structure and chemical properties to the analyte of interest. We add equal amount of IS to all samples including blank and used to calculate the analyte loss while preparing the sample. IS used for calibration by plotting the ratio of analyte signal to the IS signal.


Internal standard for melarsoprol?

yes


Why is it necessary to use an internal standard in the GLC assay?

Internal standard is primarily used to increase the accuracy and precision of analytical methods that have large inherent variability. The method is used in chromatography (GC, HPLC) where a compound similar to the analyte of interest is added to the sample and run. By having the analyte and the standard elute in the same run, the run to run variability is eliminated giving more precise results. Obviously one needs to calibrate the responses of the internal standard with that of the analyte. Incidental benefits are saving time and money by having less runs. Hope this is useful. Jay, Winnipeg, Canada


Why propyl paraben used in HPLC calibration?

propyl paraben being a stable compound with reproducible chromatographic parameters used as a internal standard for the calibration of the HPLC systems


What is standard width of internal roads?

12 feet.


What are the disadvantages of an internal standard in gas chromatography?

It has a poor efficiency for non-routine analysis. (you need to create an internal standard plot for each different ion you are measuring).


Explain the power sources in a hybrid car.?

Gasoline burned in a standard internal combustion engine.Electricity stored in a battery used to turn an electric motor.


What is the standard internal drive interface that you use today?

The most popular consumer internal drive interface is SATA.


Why internal standard use in GC?

We use internal standard for the identification of that compound which we want to know the concentration. No effect of the injection volume of sample. But Now a days Auto injector is coming very good quality, so we can control the injection volume of sample. So we do not need any internal standard. Nikhil


What are the units used to measure internal energy and heat?

Heat is also a type of energy, so energy units would be used in both cases. The standard (i.e., international) unit for all sorts of energy is the joule.