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What is the diffecence between sensitivity and detection limit?
Wiki User
∙ 16y ago
Sensitivity is the smallest change in concentration of the analyze that can be detected by using that method. This is the slope of the calibration curve.
Detection limit is the lowest concentration that can be detected by the particular method.
Wiki User
∙ 16y ago
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What is the minimum amount of protein that can appear in SDS PAGE gel?
Although this would in part depend the size of the protein being separated and stained (for some staining methods), the largest factor that determines sensitivity of SDS-PAGE is the type of staining method used: - If staining is done with coomassie brilliant blue, the limit of detection claimed by most suppliers is 50 ng. In my experience, 100-1000 ng is more accurate for proteins of 20-30 kDa. - If staining is done with silver stain, the limit of detection is much lower (or higher sensitivity). Manufacturers usually claim that 5-50 ng of protein can be visualized, but in my experience 50 ng is the lower limit for average sized proteins (20-30 kDa). - If visualization is accomplished with an enzyme immunoassay, the limit of detection is lower still, as low as 0.1-1 ng (100-1000 pg). - If visualization is accomplished with radio immunoassay the limit of detection becomes much lower again, easily to the picogram level (0.001 ng).
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ability of instrument to determine its desired elements
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As per W.H.O the limit for hardness in water is in between (60-11)mg/L....
How many numbers are there on the Richter Scale?
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What is the formula for detection limit?
3
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What is the detection limit for cobalt in AAS method?
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What is the minimum amount of protein that can appear in SDS PAGE gel?
Although this would in part depend the size of the protein being separated and stained (for some staining methods), the largest factor that determines sensitivity of SDS-PAGE is the type of staining method used: - If staining is done with coomassie brilliant blue, the limit of detection claimed by most suppliers is 50 ng. In my experience, 100-1000 ng is more accurate for proteins of 20-30 kDa. - If staining is done with silver stain, the limit of detection is much lower (or higher sensitivity). Manufacturers usually claim that 5-50 ng of protein can be visualized, but in my experience 50 ng is the lower limit for average sized proteins (20-30 kDa). - If visualization is accomplished with an enzyme immunoassay, the limit of detection is lower still, as low as 0.1-1 ng (100-1000 pg). - If visualization is accomplished with radio immunoassay the limit of detection becomes much lower again, easily to the picogram level (0.001 ng).
How do you calculate the limit of detection from a calibration curve?
See this link.
How do you determine limit of detection manually?
ii woiuld not know
How soon can you take a clean ua after use?
Under the detection limit can vary. Between 3-7 days, depending on daily amount and usage.
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Early detection of high blood pressure can limit damage to your heart and arteries!
What is the Detection limit of atomic absorption spectroscopy?
ability of instrument to determine its desired elements
Can neptune be seen with out a telescope at night?
No. Its brightness is below the limit of sensitivity for the unaided human eye.
What is the range of laser communication?
There's no set limit, it depends on the power of the laser and the sensitivity of the receiver.
