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ViviSensitivity refers to the ability of a method to detect small changes in analyte concentration, whereas the detection limit is the smallest concentration of an analyte that can be reliably detected and distinguished from background noise. In other words, sensitivity measures the response of a method to concentration changes, while the detection limit indicates the lowest concentration that can be detected with a defined level of certainty.
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How does instrument parameters affect the absorption spectrum?
Instrument parameters such as resolution, signal-to-noise ratio, and detection limit can affect the accuracy and precision of absorption spectra measurements. A higher resolution can provide more detailed peak information, while a higher signal-to-noise ratio can improve the sensitivity of detecting small absorbance changes. Additionally, a lower detection limit can allow for the detection of lower concentrations of analytes.
What is the minimum amount of protein that can appear in SDS PAGE gel?
The minimum amount of protein that can be detected in a SDS-PAGE gel generally depends on the sensitivity of the staining or detection method used. However, in general, amounts as low as 1-10 ng of protein can be detected on an SDS-PAGE gel.
What is the Detection limit of atomic absorption spectroscopy?
The detection limit of atomic absorption spectroscopy is typically in the range of parts per billion (ppb) or even parts per trillion (ppt) for certain elements. This is the lowest concentration of an element that can be reliably detected by the technique. The detection limit can vary depending on the specific element being analyzed and the instrumentation used.
Why does atomic absorption spectroscopy often have a low detection limit that atomic emission spectroscopy?
Atomic absorption spectroscopy typically has a lower detection limit compared to atomic emission spectroscopy because it measures the amount of light absorbed by atoms in a sample, which is more sensitive at low concentrations. Atomic emission spectroscopy, on the other hand, measures the intensity of light emitted by atoms, which can be affected by background noise and matrix effects, leading to a higher detection limit.
What are the validation parameters for related substance by hplc analysis?
The validation parameters for related substances analysis by HPLC typically include specificity, linearity, accuracy, precision, detection limit, quantification limit, and robustness. Specificity ensures the method can differentiate between the analyte and impurities, while linearity confirms a linear relationship between concentration and response. Accuracy and precision assess the closeness of results to the true value and the method's repeatability. Detection and quantification limits determine the lowest concentration that can be reliably detected and quantified. Robustness evaluates the method's ability to remain unaffected by small variations in parameters.
