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This solution contains the detergent sodium dodecyl sulfate (SDS)

which dissolves the cell membrane and denatures proteins. The solution is very alkaline (pH > 12) due to the presence of sodium hydroxide. The high pH aids in denaturing proteins and causes the cleavage

of the phosphate bonds in RNA. This eliminates interference from high

molecular weight RNA during the plasmid purification. Under highly

alkaline conditions, the two strands in non-supercoiled DNA (linear

fragments of chromosomal DNA, relaxed and nicked circular DNA)

separate and are partially removed from solution. However, this does

not occur with supercoiled forms of plasmid DNA because the two

strands are intertwined and entangled in a way that prevents them

from coming apart. Therefore, supercoiled plasmid remains free in

solution.

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Q: What is the function of solution 2 in plasmid isolation?
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