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The Rf (retention factor) values of amino acids can vary based on the specific conditions used in thin layer chromatography. Generally, Rf values for different amino acids will fall within a range of approximately 0.1 to 0.9, with each amino acid having a unique Rf value based on its individual chemical properties. For precise values, it is best to consult specific experimental data or reference sources.
Rf values are used mainly for simplicity. These values are generally easier to use to obtain percentages between a couple objects instead of using values that are already established.
Yes, two substances with different Rf values can still be similar. Differences in Rf values can be due to various factors, such as the polarity of the compound or the composition of the stationary phase. Therefore, substances with different Rf values can still share similar chemical structures or functional groups.
Rf values can be used to identify the type of drugs that had been taken by those who went through drug overdose.
Rf Values determine the solubility of a substance with respect to a certain solvent.
The order of increasing RF values in TLC is Benzil, methanol anthracene and tryphenyl.
Ni2+ and Fe3+ ions have different Rf values because they have different charge-to-size ratios, leading to differences in their interactions with the stationary phase in the chromatography process. The higher charge of Fe3+ compared to Ni2+ results in stronger electrostatic interactions with the stationary phase, causing it to move slower and have a higher Rf value.
RF- Radio Frequency, which is in the measure of kilo & mega Hz range
If you switch from a hexane-ethyl acetate solvent system to ethyl acetate only, you would expect the Rf values to decrease. Ethyl acetate is a more polar solvent than hexane, so compounds will interact more with the solvent and have shorter distances of travel on the TLC plate, resulting in lower Rf values.
No, the Rf values will not increase for each pigment with a longer chromatography run time. The Rf value is a constant characteristic of a compound in a particular solvent system and is not affected by the duration of the chromatography run.
Rf value to compare the "unknown" components of colored candy dyes with the "known" components of food coloring dyes.
No. The molecules MAY (and probably are if you are presented with this situation in school/college lab courses) be identical but just the Rf values is not enough information to determine, you must conduct additional tests. Two different molecules can have the same Rf value.Compound A will always have an Rf of X in solvent M. Compound B will always have and Rf of Y in solvent M. But, Rf X can be equivalent to Rf Y without compounds A and B being identical.