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Q: When enzyme activity is graphed versus the following which graph is a straight line?
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The enzyme phosphofructokinase is an important enzyme that plays a significant regulatory role in glycolysis. Which of the following conditions would be expected to result in increased activity of this enzyme?

Low levels of ATP


What can you do to regain the activity of the enzyme?

The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity


What is the effect of hydrochloric acid on the enzyme activity?

Denature enzyme activity


What are the effects of physical activity on enzyme activity?

Physical activity can alter the shape of enzyme which can cause damage or may the enzyme become inactive


What are the effects of freezing on enzyme activity?

When an enzyme is frozen, it only slows down activity. Unlike boiling an enzyme, it does not stop it from working.


What switchs on enzyme activity while what can switch off or reduce enzyme activity?

activators; inhibitors


What is the effect of cold temperatures on enzyme activity?

Cold temperatures have a drastic effect on an enzyme's activity level. Cold temperatures usually dramatically slow down an enzyme's activity.


What can change the way an enzyme acts?

Enzyme activity is affected by other molecules, temperature, chemical environment (e.g., pH), and the concentration of substrate and enzyme. Activators are molecules that encourage enzyme activity, and inhibitors are enzymes that decrease enzyme activity. Sometimes a cofactor is necessary for the enzyme to work.


Which blocks enzyme activity by binding to the site of an enzyme?

inhibitor


What accurately describes how temperature affects enzyme activity?

Enzyme activity increases with temperature, but only up to a point.


Why is protein concentration necessary for accurate comparison of the enzyme activity of the fractions?

Enzyme activity sometimes reflects the amount of protein expressed in a cell--however, due to enzyme inhibitors, the enzyme activity is not always reflective of the amount of protein expressed by a cell.


What determine an enzymes activity?

There are several things that determine an enzyme's activity. The main determinants include the structure of the enzyme, temperature, pH and so much more.