All agar plates are incubated at all temps because that is the best temperature for those particular bacteria to grow and reproduce quickly so a person can see results. Some agar plates get incubated at room temp around 22-24c so only certain bacteria will grow, or 30c so only yeast will grow or 37c so only E. coli will, the temp depends on what bacteria is being grown.
Because 35C is close to normal body temperature of 37C
5.72 million red blood cells per microliter (mcL) of blood for men and 5.03 million per mcL for women. In healthy men, 41 percent to 50 percent of blood is red blood cells. In healthy women, 35 percent to 46 percent is red blood cells
Composition * Dehydrated powder, hygroscopic in nature, store in a dry place, in tightly-sealed containers below 25°C and protect from direct Sunlight. Instructions for Use Dissolve 45gms in 445 ml distilled water. Gently heat to boiling with gentle swirling and dissolve the medium completely. Sterilize by autoclaving at 15 psi (121°C) for 15 minutes. Cool to 45-50°C. Aseptically add equal amount (445ml) of sterile 2% Haemoglobin powder (TS 021) and add 10ml of Vitamins growth supplement (TS 022) or Yeast autolysate supplement (TS 023). Mix well. Dispense into sterile Petri plates or sterile culture tubes. Appearance: Light amber color, clear to slightly opalescent gel. On addition of Haemoglobin: Chocolate brown colour appears, opaque gel pH (at 25°C): 7.3 ± 0.2 Principle CHOCHOLATE AGAR BASE is used for isolation and cultivation of fastidious microorganisms like Neisseria gonorrhoeae. It can also be used for cultivation of aerobic, anaerobic and microaerophillic microorganisms. Medium contains Proteose peptone is a nitrogen source required for the growth of wide variety of organisms. Dextrose acts a carbon energy source. Disodium phosphate buffers the medium whereas sodium chloride maintains the osmotic equilibrium. Agar is the solidifying agent. This medium is supplemented with cofactor, which provides NAD to facilitate the growth of Haemophilus influenzae, Neisseria gonorrhoeae and Neisseria meningitidis. Heated sheep blood is added to give the medium its "chocolate" appearance. This medium is prepared, stored and dispensed under oxygen-free conditions to prevent the formation of oxidized products prior to use. Interpretation Cultural characteristics observed after inoculating (103CFU/ml), on incubation at 35 ± 2°C for 48 hours with CO2. Ingredients Gms/Ltr. Proteose peptone 20.0 Agar 15.0 Sodium chloride 5.00 Disodium phosphate 5.00 Dextrose 0.50 Regards NITIN MALHOTRA
In humans and other mammals, hemoglobin makes up about 97% of a single red blood cell's dry content, and around 35% of the total content (including water)
If a 32-year old male is in the AB plus blood group, he can marry a 35-year old female that is in the O blood group without having any problems in pregnancy.
120
Agar is a medium, so you are checking the sterility of the agar. After preparation one usually places an agar plate at room temperature and another agar plate at 35 to 37 degrees C. After 24-48 hrs of incubation a visual check is made to see if there is any visible growth on the uninoculated plates. If you are adding blood, etc. to the agar, those components can be checked by subbing them to a blood agar plate to see if there is any growth--which would indicate non-sterile components.
To determine that an agar plate is sterlize you can keep it overnight in incubator without streaking and if there is any growth peresent in it then it is contaminated and if not then it is obveiously sterlize
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The pressure of blood returning to the heart; 35 is low.
Plates for sleighing are roughly $30 - $35.
384,475,000 license plates. There are 35 different letter/number combinations possible. Each combination has 10,985,000 variants. 35*10,985,000 = 384,475,000
.35
1st Look - 2008 Young Blood 1-35 was released on: USA: 8 August 2009
Normal CO2 in the blood is the same as Normal PH. Ph is 7.35-7.45 CO2 is 35-45
110/80
they are now only manafactured in china as they were banned everywhere else as they contained lead
Mr- and Mrs- North - 1952 Salt in His Blood 1-35 was released on: USA: 29 May 1953