Whether it's staining, cultures or magnification we all go by the motto "less is more". You start with the lower power magnifications to position the plate and increase power until you get a clear view.
3200x
650 x
As you change objectives from 4x to 10x to 40x, the field of view will decrease. This is because higher magnification objectives bring smaller areas into focus, resulting in a narrower field of view.
The magnification of a microscope is calculated by multiplying the magnification of the objective lens by the magnification of the eyepiece. In this case, if you have a 10x low power objective and a 10x high power objective, the total magnification would be 100x (10x * 10x) for both objectives when used with the same eyepiece magnification.
The magnification of the microscope depends on the objectives used. To calculate the total magnification, multiply the magnification of the eyepiece (10x) by the magnification of the objective lens being used. If you had two objectives, each with magnifications of, for example, 40x and 100x, the total magnification would be 400x and 1000x respectively when using the 10x eyepiece.
The highest magnification would be achieved by using the 43x objective lens with the 10x eyepiece lens, resulting in a total magnification of 430x (43 x 10 = 430).
Using 5x oculars instead of 10x will result in a lower total magnification for the microscope system. The magnification formula for microscopes is the product of the magnification of the ocular lens and the objective lens. Therefore, with 5x oculars, you will achieve half the total magnification compared to using 10x oculars with the same objectives.
When using a microscope, you typically start with the lowest power objective, often the 4x or 10x objective. This allows for a wider field of view and makes it easier to locate your specimen. Once the specimen is in focus at low power, you can then switch to higher power objectives for more detailed observation. Always ensure to adjust the fine focus when transitioning to higher magnifications to avoid damaging the slide or the lens.
Microscope objective lenses typically have magnification levels ranging from 4x to 100x. The magnification power is usually marked on the objective lens itself. Higher magnifications require the use of immersion oil to achieve clearer images.
Low power objectives on a microscope typically have magnification levels of 4x or 10x and are used for locating and centering the specimen. High power objectives, on the other hand, have magnifications ranging from 40x to 100x and are used for detailed examination of the specimen.
The objectives are what magnify an item. They are usually 10x, 40x, and 100x. These are also known as low power, high-dry, and oil immersion. Some microscopes also have a 4x for quick scanning.
I assume you mean the lenses. They're the 10x, 40x, 100x etc. They're the things you turn to see the slide better