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limiting dilution is a method used to have a single cell / well in a tissue culture plate. and why we need a single cell / well ...to ensure that we have single clone of antibody producing B cell as during any immuneresponse a mixed of different B clone will produce to make antibodies against foreign antigen even during the 2ndary immune response the antibodies produced will have different structure in the variable region because of somatic hypermutation process during affinity maturation at the germinal center. Thus, we do a limiting dilution to study each B cell clone antibody produced individually and test its specificity by ELISA, which can be easy to detect the best affinity binder clone and chose it for further steps.
What else can I help you with?
Different types of fusion technique used in hybridoma technique?
The different types of fusion technique used in Hybridoma formatio are: 1. fusion mediated by PEG. 2. fusion mediated by DMSO. 3. Electrofusion 4. Viral Transformation (viral mediated fusion) using EB Virus, Sandai Virus.
What is the formula used to calculate dilution ratios?
A dilution ratio is normally used for a mixture of two fluids: an active component and a carrier solvent. The dilution ratio is the ratio of the volume of the solvent to the volume of the active component.
Why you re using peg in hybridoma?
PEG (polyethylene glycol) is used in hybridoma technology primarily to promote the fusion of myeloma cells and antibody-producing B cells. It facilitates the merging of cell membranes, leading to the creation of hybrid cells that can produce specific monoclonal antibodies. Additionally, PEG helps to increase the yield of viable hybrid cells by promoting cell viability during the fusion process. Overall, its use enhances the efficiency and success rate of generating hybridomas.
How are antibodies made in the lab?
Antibodies are made in the lab through a process called hybridoma technology. This involves fusing a specific type of immune cell with a cancer cell to create a hybrid cell that can produce antibodies. These antibodies can then be harvested and used for various purposes, such as in diagnostic tests or as treatments for diseases.
Why is the term limiting used to describe the limiting reactant?
The term "limiting" is used to describe the reactant that is completely consumed in a chemical reaction, thus limiting the amount of product that can be formed. It determines the maximum amount of product that can be produced based on its stoichiometry and quantity.
Definition of dilution and dilution factor?
In chemistry and biology, the dilution factor is the total number of unit volumes in which the material is dissolved. As I understand it, the dilution refers to the dilution ratio. If you add 1 part of something to 4 parts of something else, the dilution ratio is 1 to 4. The dilution factor counts all the parts and expresses the same thing as 1 out of 5.
What is the key idea to remember when considering the dilution of a solution?
The key idea to remember when considering the dilution of a solution is that the amount of solute remains constant before and after dilution. Therefore, the concentration of the solute decreases as more solvent is added. The equation C1V1 = C2V2 is commonly used to calculate the new concentration or volume after dilution.
What is the reactant called that gets used up first?
The Limiting Reactant is the reactant that runs out first in a reaction.
In the calculation trypan blue exclusion assayof haemocytometer why dilution factor 2 is used not 0.5?
Because you probably used a 2-fold dilution. So you need to multiply your count by 2 to get the ACTUAL amount.
What is a Dilution Test?
A dilution test is a procedure used to measure the concentration of a substance in a solution by systematically diluting the solution and observing the impact on the concentration. This test helps to determine the original concentration of the substance by comparing it with the concentration after dilution.
Why peptone water is used in the dilution technique?
Peptone water is used in the dilution technique because it provides a nutrient-rich environment that supports the growth of bacteria. This allows for easier detection and enumeration of bacteria present in samples. Peptone water also helps maintain bacterial viability during dilution procedures.
How can one create monoclonal antibodies?
Monoclonal antibodies are created by fusing a specific type of immune cell, called a B cell, with a cancer cell to form a hybrid cell called a hybridoma. This hybridoma cell can then produce large quantities of identical antibodies that target a specific antigen. These monoclonal antibodies can be used for various medical purposes, such as diagnosing and treating diseases.
