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Q: Why should a graph of absorbance versus concentration go through the origin?
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Why should the y intersect of any Beer's law plot equal zero?

Beer's law says that absorbance is directly proportional to concentration. So if there is zero concentration there should be zero absorbance (0,0).


What is absorbance vs concentration?

Absorbance refers to a measure of the capacity associated with a substance as regards absorption of light of a specified wavelength. Whenever you plot a graph of absorbance vs. concentration a direct relationship should be produced


Why should the concentration of sample be low in spectrophotometer readings?

When doing reading on a spectrophotometer, the sample being studied is either a color change or a precipitated compound, depending on the wavelength that it is being read. If it is a precipitated compound and it has a very high concentration, then you run the risk of the light being used to measure the absorbance not going through. In which case you have total absorbance but it is inaccurate in helping you determine the concentration of your sample because you are unsure where the concentration limit is for that wavelength, and your sample could possibly be able to absorb more. In which case you still can't calculate the concentration of the sample.


Why should an absorbance level not exceed a value of 1?

Since the absorbance level is actually just a fraction of radiation absorbed at a given wavelength, it should never rise above %100.


What is the actual absorbancy if 2mL of a culture is diluted by adding 4mL of water and the absorbancy reading of the diluted culture is 0.059?

If you multiple your absorbance by the dilution factor, this should give you the absorbance of the original culture.


How do you determine molar absorptivity of cobalt nitrate?

Utilizing the Beer-Lamber Law you have A=abc here A= is the absorbance at a set wavelength a= the molar absorbtivity b= the path length c= concentration in molar The best way to determine a is to make solutions of known concentrations of cobalt nitrate (3-5 would be best) and determine the absorbance of each solution. Next plot the Abs vs concentration of each solution using something like excel or R. Determine the line of best fit ( it's important to force fit this line through 0) the R-sqr value should be no less than .95 Since the equation of a line is : y=mx +b, this is equivalent to A=abc noting that b is assumed to be 1cm we habe A=ac, where m=a and x=c In short the slope of the line of best fit in the molar absorbtivity


How much concentration is required in driving?

Driving should have your full concentration if possible.


Should the line of a normal force vs static friction graph pass through the origin Same goes for kinetic friction graph?

no


What concentration of should should be used for intravenous drip?

0.9


Strength of a chemical?

Im doing the crossword aswell :D the answer should be CONCENTRATION, good luck :D


How to fix concentration of RS by HPLC?

Concentration of sample= estimated LOQ concentration (µg/mL) x 1/desired LOQ (%) x 100LOQ should be Equal to or less than 0.05% of that of test concentration. Response of the impurity should be NLT 2000 at LOQ level for better precision.


How do you calibrate for HPLC?

standards are run with samples i.e. several solutions of chemical you are trying to analyse for, of known composition and strengths are run to set up a calibration curve which should be a straight line - absorbance (or signal strength) vs. conc. You then test the unknown sample and can extraploate the concentration of the sample based on your calibration curve. HPLC columns come with a standard chromatogram when purchased so a run with same conditions and sample should give similar retention times.