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Electric charge can be positive or negative both on large scales and fundamentally (eg the protons in atomic nuclei have a charge of +1 and the electrons that orbit those nuclei have a charge of -1 [units are arbitrary, if you want derived SI then these charges are +- 1.6*10^-19 C]).

You can also think of magnetic north and south poles as being 'positive' and 'negative' although this isn't strictly true.

Mathematically of course there are positive and negative numbers where the number -x is defined by the fact that x+(-x)=0.

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