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The addition of 5% CO2 in tissue culture creates a more physiologically relevant environment for cell culture by maintaining the pH of the culture medium and promoting cell growth. This level of CO2 represents a balance between providing enough CO2 for cellular respiration and avoiding excessive acidification of the medium.

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Q: Why use 5 percent CO2 in tissue culture?
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What concentration of alcohol is best to use for sterilization during plant tissue culture. 70 percent alcohol or absolute alcohol?

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