maximum spacing assording to IS 456 = 300mm
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A civil engineer will determine the size, spacing, oreintation, strength requirements, and coatings on the reinforcement. These are based upon the calculations of various loads and moments on the structure you are reinforcing and then applying a factor of safety.
2" based strictly on the slab thickness. However, it may be governed by form spacing, rebar spacing or rebar clearance to the forms.
Allowable clearing spacing of bars = 3d where d is depth suppose d is 180 mm than clearing spacing is 3(180) =540mm note that clearing spacing of slab donot exceed upto 750mm
Spacing for floor joists is usually determined by building codes for the location