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That is a bit of a trick question since sin could not have existed for humans prior to creation. In the alternative, Lucifer lead the heavenly rebellion against God, which can be viewed as the "original sin" resulting in the ever present battle between good and evil (God & Lucifer) and the establishment of the free-will concept as a means of fair-play between God and Lucifer.

Taken in that view, sin can be said to have had the greatest impact on creation since God created us for the sole purpose to participate in a (free-will) Chess game of sorts with Lucifer.

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