The Eid50, or effective infectious dose for 50% of a population, is calculated using dose-response data, typically obtained from experiments involving a range of doses administered to a population. You plot the proportion of individuals infected or affected against the logarithm of the dose, and then fit a sigmoidal curve to the data. The Eid50 is the dose at which 50% of the population exhibits the response or infection. Statistical software or models, such as the probit or logit model, can be used to analyze the data and determine the Eid50 value.
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