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How do you calculate total phenolics by folin ciocalteu method?
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How do you prounced folin-ciocalteu?
The word "folin-ciocalteu" refers to the scientific phenol reagent named after the two scientists, Folin and Ciocalteu. These names are of Swedish and Romanian origin. The combination is pronounced similar to "Faux-lean-cho-cal-the-oh."
What is the reaction mechanism of the folin ciocalteu reagent and poly phenols?
The Folin-Ciocalteu reagent reacts with polyphenols through a redox reaction, where the phenolic hydroxyl groups are oxidized by the reagent, forming a blue complex. This reaction is typically followed by a colorimetric measurement to quantify the total phenolic content present in the sample.
How folin-ciocalteu works in total phenolic content method?
Folin-Ciocalteu reagent reacts with phenolic compounds to form a blue-colored complex, which can be measured spectrophotometrically. The intensity of the color is directly proportional to the total phenolic content present in the sample. By comparing the absorbance of the sample to a standard curve of known concentrations, the total phenolic content of the sample can be quantified.
How folin ciocalteu works in total phenolic content method?
Folin's method measures -OH groups in a sample based on the fact that light absoption increases as OH groups in a sample increases. So, this method do not measure the exact amount of polyphenols because polyphenols are not the only compound has OH group if other OH group containing compounds are in the same medium such as protein, carbohyrate, vitamin C, and saponin.
Chemical tests for flavonoids?
Common chemical tests for flavonoids include the Shinoda test, Folin-Ciocalteu test, and the sodium hydroxide test. The Shinoda test involves the addition of magnesium in the presence of concentrated hydrochloric acid, resulting in color changes. The Folin-Ciocalteu test uses a phenol reagent to detect flavonoid presence based on color development. The sodium hydroxide test involves the addition of sodium hydroxide solution to a flavonoid-containing sample to observe color changes.
What are the reagents used in the estimation of antioxidants?
Common reagents used in the estimation of antioxidants include DPPH (2,2-diphenyl-1-picrylhydrazyl) reagent, Folin-Ciocalteu reagent, and Trolox standard. These reagents are utilized in assays such as DPPH radical scavenging assay, total phenolic content assay, and oxygen radical absorbance capacity (ORAC) assay to measure the antioxidant capacity of compounds.
How to make in laboratory Folin and Wu's solution?
A description of the method and reagents is at this link.
Why protein estimation is done at 660 nanometer in folin lowry method?
Protein estimation in the Folin-Lowry method is done at 660 nanometers because this wavelength corresponds to the highest absorption peak of the complex formed between proteins and the Folin-Lowry reagent. This wavelength is ideal for accurately measuring the concentration of proteins present in a sample based on the colorimetric reaction that occurs.
When did Otto Folin die?
Otto Folin died on 1934-10-23.
When was Otto Folin born?
Otto Folin was born on 1867-04-04.
When did Léopold de Folin die?
Léopold de Folin died in 1896.
When was Léopold de Folin born?
Léopold de Folin was born in 1817.
