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Standard curves are used to determine the concentration of substances. First you perform an assay with various known concentrations of a substance you are trying to measure. The response might be optical density, luminescence, fluorescence, radioactivity or something else. Graph these data to make a standard curve - concentration on the X axis, and assay measurement on the Y axis.
Also perform the same assay with your unknown samples. You want to know the concentration of the substance in each of these unknown samples.
To analyze the data, fit a line or curve through the standards. For each unknown, read across the graph from the spot on the Y-axis that corresponds to the assay measurement of the unknown until you intersect the standard curve. Read down the graph until you intersect the X-axis. The concentration of substance in the unknown sample is the value on the X-axis.
In the example below, the unknown sample had 1208 counts per minute, so the concentration of the hormone is 0.236 micromolar.
Prism makes it very easy to fit your standard curve, and to read (interpolate) the concentration of unknown samples.
What else can I help you with?
Is it true that The smaller the standard deviation of a normal curve the higher and narrower the graph?
Yes, that's true. In a normal distribution, a smaller standard deviation indicates that the data points are closer to the mean, resulting in a taller and narrower curve. Conversely, a larger standard deviation leads to a wider and shorter curve, reflecting more variability in the data. Thus, the standard deviation directly affects the shape of the normal distribution graph.
What kind of curve does the graph show?
depends what graph....
Show you curve graph?
i need a graph of some sort for a essay
What shows the speed on a distance-time graph?
The slope of the graph line or curve.
What happens to the graph of a normal curve as the mean decreases?
The graph moves to the left.
How do you prepare the standard curve?
To prepare the standard curve you will need linear graph paper, semi-log graph paper and absorbance. You can define your standard curve by finding the absorption or percent plot on the Y axis.
Is it true that The smaller the standard deviation of a normal curve the higher and narrower the graph?
Yes, that's true. In a normal distribution, a smaller standard deviation indicates that the data points are closer to the mean, resulting in a taller and narrower curve. Conversely, a larger standard deviation leads to a wider and shorter curve, reflecting more variability in the data. Thus, the standard deviation directly affects the shape of the normal distribution graph.
What shape is a Cooling curve graph?
A Cooling curve graph changes shape.
What kind of graph is a bell shaped curve?
The bell curve graph is another name for a normal (Gaussian) distribution graph. A Gaussian function is a certain kind of function whose graph results in a bell-shaped curve.
What kind of curve does the graph show?
depends what graph....
What is the indefference curve?
Indifferent curve is a graph. a graph that shows the difference on the sales of two products or commodities...
Is the graph of a curve a function?
The graph if a function can be a curve, but it can also be any one of a ton of other shapes.
What is indefferent curve?
Indifferent curve is a graph. a graph that shows the difference on the sales of two products or commodities...
How does the standard curve work in Biology?
Biologists assume that the systems they are studying are "representative" of the population as a whole, that is, that the population's test results will be distributed according to the standard curve...ie, that graph of scores that best covers the range of 90% of scores in the entire population. In short, the standard curve provides the baseline by which to compare test results.
Where is profit maximized on a graph?
Profit is maximized on a graph where the marginal cost curve intersects the marginal revenue curve.
How do you draw a momentum -time graph for force-time graph?
The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.
What shaped graph is associated with polygenic inheritance?
The shaped graph that is associated with polygenic inheritance is a bell curve graph. The majority of these graphs are also combined with a bar graph under the curve.
