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Standard curves are used to determine the concentration of substances. First you perform an assay with various known concentrations of a substance you are trying to measure. The response might be optical density, luminescence, fluorescence, radioactivity or something else. Graph these data to make a standard curve - concentration on the X axis, and assay measurement on the Y axis.
Also perform the same assay with your unknown samples. You want to know the concentration of the substance in each of these unknown samples.
To analyze the data, fit a line or curve through the standards. For each unknown, read across the graph from the spot on the Y-axis that corresponds to the assay measurement of the unknown until you intersect the standard curve. Read down the graph until you intersect the X-axis. The concentration of substance in the unknown sample is the value on the X-axis.
In the example below, the unknown sample had 1208 counts per minute, so the concentration of the hormone is 0.236 micromolar.
Prism makes it very easy to fit your standard curve, and to read (interpolate) the concentration of unknown samples.
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depends what graph....
Show you curve graph?
i need a graph of some sort for a essay
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The graph moves to the left.
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What kind of curve does the graph show?
depends what graph....
What is indefferent curve?
Indifferent curve is a graph. a graph that shows the difference on the sales of two products or commodities...
What is the indefference curve?
Indifferent curve is a graph. a graph that shows the difference on the sales of two products or commodities...
Is the graph of a curve a function?
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How does the standard curve work in Biology?
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How do you draw a momentum -time graph for force-time graph?
The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.
What shaped graph is associated with polygenic inheritance?
The shaped graph that is associated with polygenic inheritance is a bell curve graph. The majority of these graphs are also combined with a bar graph under the curve.
Why is the Graph of the Indifference Curve Shaped like lower curve?
yes
What is a bell curve?
A bell curve is a symmetrical bell-shaped graph curve that represents the arrangement of values, how often something occurs, or probability of occurance of a set of data. It slopes downward from a point in the middle corresponding to the average also called mean value, or the maximum probability. Gather all the results of an IQ test and make a graph of them, would create a bell curve with average IQ being the mean value. The more results or values, the better the bell curve. It can be made with a line graph or a bar graph.
