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Standard curves are used to determine the concentration of substances. First you perform an assay with various known concentrations of a substance you are trying to measure. The response might be optical density, luminescence, fluorescence, radioactivity or something else. Graph these data to make a standard curve - concentration on the X axis, and assay measurement on the Y axis.
Also perform the same assay with your unknown samples. You want to know the concentration of the substance in each of these unknown samples.
To analyze the data, fit a line or curve through the standards. For each unknown, read across the graph from the spot on the Y-axis that corresponds to the assay measurement of the unknown until you intersect the standard curve. Read down the graph until you intersect the X-axis. The concentration of substance in the unknown sample is the value on the X-axis.
In the example below, the unknown sample had 1208 counts per minute, so the concentration of the hormone is 0.236 micromolar.
Prism makes it very easy to fit your standard curve, and to read (interpolate) the concentration of unknown samples.
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Show you curve graph?
i need a graph of some sort for a essay
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What kind of curve does the graph show?
depends what graph....
What is indefferent curve?
Indifferent curve is a graph. a graph that shows the difference on the sales of two products or commodities...
What is the indefference curve?
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How does the standard curve work in Biology?
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Where is profit maximized on a graph?
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How do you draw a momentum -time graph for force-time graph?
The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.The momentum-time graph is the integral of the force-time graph. that is, it is the area under the curve of the f-t graph.
How can one calculate the unknown concentration from a standard curve?
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What shaped graph is associated with polygenic inheritance?
The shaped graph that is associated with polygenic inheritance is a bell curve graph. The majority of these graphs are also combined with a bar graph under the curve.
