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Q: How do you prepare 10mM b mercaptoethanol?
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How do you prepare 1M from 10mM Tris-HCL?

To prepare 1M Tris-HCl from a 10mM solution, you would need to dilute the 10mM solution by a factor of 100. This means you would mix 1 part of the 10mM solution with 99 parts of water to achieve a final concentration of 1M Tris-HCl.


What is the role of 2- mercapto ethanol in RNA extraction?

2-mercaptoethanol is used in RNA extraction to inactivate RNases, which are enzymes that can degrade RNA. By adding 2-mercaptoethanol to the extraction buffer, it helps to maintain the integrity of RNA by denaturing and deactivating RNases present in the sample. This ensures that the RNA obtained from the extraction process is of high quality and suitable for downstream applications.


What is the chemical formula of 2-mercaptoethanol?

C2H6OS


What is the role of 2-mercaptoethanol in plant protein extraction?

2-mercaptoethanol is a reducing agent that helps break disulfide bonds in proteins, allowing for better extraction of plant proteins. By disrupting these bonds, 2-mercaptoethanol helps to solubilize proteins and prevent their aggregation during the extraction process.


What is another solvent that can be used instead of beta-mercaptoethanol?

dithiothreitol


How do you prepare 10mM Tris Hcl?

1.21 g Tris-HCl, QS water to 1L. Scale appropriately.


How do you prepare 10mM ferric chloride solution?

To prepare a 10mM ferric chloride solution, first calculate the molecular weight of FeCl3 to determine the amount needed to achieve a concentration of 10mM. Dissolve this amount in a known volume of water or solvent to make up the final volume of the solution, ensuring thorough mixing to achieve homogeneity.


What is the role of 2-mercaptoethanol in the isolation of genomic DNA from a plant source?

It is an antioxidant.


What is the role of beta-mercaptoethanol in DNA extraction?

stabilization of phenol against oxidation


How do you prepare 10mM solution of Tris-HCl?

To prepare a 10mM solution of Tris-HCl, you would weigh out the appropriate amount of Tris-HCl powder using a balance and dissolve it in water to make a final volume of solution. For example, to make 1L of 10mM Tris-HCl solution, you would need to dissolve 0.121g of Tris-HCl in 1L of water.


How do you prepare 10mM Tris?

To prepare 10mM Tris solution, first calculate the amount of Tris base needed based on the molecular weight of Tris (121.14 g/mol). Weigh out the appropriate amount of Tris base and dissolve it in water to make a final volume of 1L. Adjust the pH to the desired value if necessary.


Why you are using 2 mercaptoethanol in mes cell culture?

Mercaptoethanol is a thiol compound that is involved in the reduction of substances and prevents the oxidation of cysteine to cystine, thus increasing cysteine activity. It acts as an antioxidant by itself and indirectly by increasing intracellular gluthathione and cysteine activity