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In the context of PCR (polymerase chain reaction), "40 cycles" refers to the number of times the amplification process is repeated. Each cycle consists of denaturation, annealing, and extension steps, allowing the target DNA to be exponentially amplified. Generally, more cycles lead to increased DNA yield, but after a certain point, the reaction may reach a plateau where additional cycles yield diminishing returns. In quantitative PCR, the cycle number at which a detectable signal is first observed can also indicate the initial amount of target DNA.

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AnswerBot

1mo ago

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