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Free coagulase is an extracellular toxin that reacts with a thrombin-like, thermostable substance known as coagulase-reacting factor (CRF) or serum factor. The reaction of coagulase and CRF will lead to the formation of coagulase-CRF complex where this complex indirectly changes frbrinogen to fibrin (clotting factor) and hence will form a fibrin clot. Furthermore, it is also use as the primary indicator of virulence among staphylococci. Its clinical significance is that when doing the coagulase tube test, the free coagulase will react with the plasma in the tube and hence convert the fribrinogen into fibrin which lead to fibrin clot formation in the tube. This is shown after one day of incubation of the tube, when the tube being invert, the plasma will not go down showing that it is clotted.

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Q: What is a free coagulase and its significance?
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What is difference between tube coagulase and slide coagulase test?

The tube coagulase test detects the presence of bound coagulase enzyme in the plasma and is considered more specific. The slide coagulase test detects free coagulase enzyme released extracellularly and is considered less specific. Both tests are used to differentiate Staphylococcus aureus from other staphylococci.


Is coagulase test a microbiological test?

Yes, the coagulase test is a microbiological test used to determine the presence of the enzyme coagulase produced by some Staphylococcus bacteria. It helps differentiate between coagulase-positive Staphylococcus aureus and coagulase-negative staphylococci.


What are 2 types of staphylococal coagulase?

2 types of staphylococal coagulase are 1.staph.epidermidis/albus2.staph.aureus


What is a coagulase test used for?

The coagulase test is used to differentiate between Staphylococcus aureus (coagulase positive) and other Staphylococcus species (coagulase negative). Staphylococcus aureus is a pathogenic bacterium, while other species are often less virulent. The test involves detecting the ability of the bacterium to coagulate plasma by producing the enzyme coagulase.


What is the operator broth test?

The operator broth test, also known as the coagulase test, is a microbiological test used to differentiate between Staphylococcus aureus (positive coagulase reaction) and other species of Staphylococcus. It involves adding a coagulase reagent to a bacterial culture and observing for clot formation to determine the presence of the enzyme coagulase.


Does bacilus subtilis test negative for coagulase test?

Yes, Bacillus subtilis is negative for the coagulase test. Coagulase is an enzyme produced by some bacteria (such as Staphylococcus aureus) that causes blood plasma to clot, resulting in a positive test. Bacillus subtilis does not produce coagulase, so it will not clot the plasma and will have a negative test result.


Why is it important to use fresh cultures in a coagulase test?

Using fresh cultures in a coagulase test is important to obtain accurate results. The presence of viable and active bacteria in a fresh culture ensures that the enzyme coagulase is active and able to induce clotting of plasma, which is necessary for a positive test result. Using old or expired cultures may lead to false negative results due to decreased enzyme activity.


What is a CNS and what do they do?

coagulase-negative staphylococci (CNS)


What makes coagulase important for a pathogen?

Coagulase is important for pathogens because it promotes blood clotting, which helps the pathogen evade the host immune response and wall off the infection. By forming blood clots, coagulase helps the pathogen establish a protected niche within the host where it can multiply and survive.


What is bound coagulase?

Bound coagulase is an enzyme produced by some strains of Staphylococcus aureus that is bound to the bacterial cell wall. It interacts with fibrinogen present in blood plasma to form a clot, aiding in the evasion of the host immune response. This enzyme is a key virulence factor for Staphylococcus aureus in causing infections.


Micrococcus luteus produces the enzyme coagulase?

Micrococcus luteus does not produce coagulase. Coagulase is typically produced by Staphylococcus aureus. Micrococcus luteus is a Gram-positive bacterium that is commonly found in soil and on human skin.


What does the enzyme coagulase do?

Coagulase is an enzyme produced by some strains of Staphylococcus bacteria that promotes blood clotting by converting fibrinogen to fibrin. This ability helps the bacteria evade the host immune response by forming protective clots around themselves.