Line segment
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Perpendicular bisector.
A bisector is a line (or line segment) which passes through the midpoint. You can have multiple lines intersect at this one point, and all of them will bisect the original line segment, since they pass through its midpoint. A perpendicular bisector passes through the midpoint, and also is perpendicular to the original line segment, so there will be only one of those.
Since there is no such word as "perpindicuar", it is difficult to be sure. A line segment can have only one perpendicular bisector.
Exactly one. No more, no less.
Altitude.