Saliva sample testing is a technique used to collect samples of a person's saliva, or spit, to check for or monitor certain drugs, hormones (chemical messengers from one cell or group of cells to another), antibodies
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Smoking, eating, drinking, and brushing the teeth can affect test results, as can the way in which the person puts saliva on the slide.
At-home kits that use saliva instead of urine to help determine ovulation have made it more convenient for women trying to conceive children to track their hormonal cycles, eliminating a lot of guesswork.
A small piece of something taken for testing or analysis.
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It is an assumption to hypothesis testing. I can not comment on the significance of a violation of these assumptions without knowing how the non-random sample was taken.