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The formation of a tripeptide involves the condensation of three amino acids through peptide bonds. The general reaction can be represented as:

Amino Acid 1 + Amino Acid 2 + Amino Acid 3 → Tripeptide + 2 H₂O

In this process, the carboxyl group of one amino acid reacts with the amino group of another, releasing water molecules and forming a peptide bond between the amino acids.

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