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Q: What is the function of exonuclease?
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What is 3'5' exonuclease activity?

3'5' exonuclease activity refers to an enzyme's ability to degrade DNA or RNA molecules by removing nucleotides one at a time from the 3' to the 5' end of the strand. This type of exonuclease activity is important in proofreading and repairing DNA replication errors.


What is the enzyme that opens up DNA?

restriction endonuclease and exonuclease


What type of biological molecule is an exonuclease?

An exonuclease is an enzyme that hydrolyzes nucleotides from the end of a nucleic acid chain. It is a type of protein, which is a biological macromolecule responsible for catalyzing biochemical reactions in living organisms.


What is the difference between an endonuclease and an exonuclease?

An endonuclease cleaves nucleic acids internally at specific recognition sites, while an exonuclease cleaves nucleic acids at the ends by removing nucleotides one at a time. Endonucleases are involved in processes like DNA repair and recombination, while exonucleases are important for proofreading during DNA replication.


What is is a linker?

The DNA found between nucleosomes on chromatin; since it is not complexed to proteins as strongly as other forms of dna, it is accessible to exonuclease hydrolysis.


What is the difference between DNA polymerase III and DNA polymerase I?

pol 1 - exonuclease activity pol 2 - dna repair pol 3 - primary replication enzyme


What are enzymes that have a proofreading role in DNA replication?

DNA polymerases, such as DNA polymerase III in prokaryotes and DNA polymerase delta in eukaryotes, have proofreading activities during DNA replication. These enzymes possess exonuclease activity, allowing them to detect and correct errors in newly synthesized DNA strands by removing misincorporated nucleotides. This proofreading function helps maintain the fidelity of DNA replication.


Polymerase 1 without 5' to 3' activity is called?

Polymerase 1 without 5' to 3' activity is called exonuclease. It is responsible for removing RNA primers during DNA replication.


How can one differentiate between single strand DNA and double strand DNA?

One can differentiate between single-strand DNA and double-strand DNA by treating with exonuclease I which specifically digests only ssDNA. Note. For verification, the products have to be run on polyacrylamide gels with appropriate controls. Hope this helps One can differentiate between single-strand DNA and double-strand DNA by treating with exonuclease I which specifically digests only ssDNA. Note. For verification, the products have to be run on polyacrylamide gels with appropriate controls. Hope this helps


Difference Between 3' to 5' and 5' to 3' Exonuclease Activity?

3' to 5' exonuclease activity refers to the ability of an enzyme to degrade DNA from the 3' end towards the 5' end, while 5' to 3' exonuclease activity degrades DNA in the opposite direction, from the 5' end towards the 3' end. These activities are important in processes like DNA repair, proofreading during DNA replication, and removal of RNA primers during DNA synthesis.


What is a linker DNA?

Linker DNA is short stretches of DNA that connect nucleosomes, the basic units of chromatin. These linkers help to position and stabilize nucleosomes along the DNA strand and play a role in regulating gene expression and chromatin structure.


What is the difference between Restriction endonucleases and exonucleases?

Exonuclease enzymes cleave nucleotides from the ends of DNA molecules. Endonuclease enzymes cleave a phosphodiester bond somewhere within the DNA molecule (not at the ends).