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Is exonuclease activity 3-5 or 5-3?
Exonuclease activity can occur in both 3' to 5' and 5' to 3' directions, depending on the specific enzyme involved. 3' to 5' exonucleases remove nucleotides from the end of the DNA or RNA strand at the 3' end, while 5' to 3' exonucleases remove nucleotides from the 5' end. These activities play crucial roles in DNA repair, replication, and degradation processes.
Is the relation a function?
Not every relation is a function. But every function is a relation. Function is just a part of relation.
The function f(x) x3 is called the function?
The cubic function.
If an inverse function undoes the work of the original function what does the original function's becomes the inverse function's domain?
Range
What are the ways in describing a function?
A formula or graph are two ways to describe a math function. How a math function is described depends on the domain of the function or the complexity of the function.
Does RNA polymerase have exonuclease activity during transcription?
No, RNA polymerase does not have exonuclease activity during transcription.
What is 3'5' exonuclease activity?
3'5' exonuclease activity refers to an enzyme's ability to degrade DNA or RNA molecules by removing nucleotides one at a time from the 3' to the 5' end of the strand. This type of exonuclease activity is important in proofreading and repairing DNA replication errors.
What is the enzyme that opens up DNA?
restriction endonuclease and exonuclease
What type of biological molecule is an exonuclease?
An exonuclease is an enzyme that hydrolyzes nucleotides from the end of a nucleic acid chain. It is a type of protein, which is a biological macromolecule responsible for catalyzing biochemical reactions in living organisms.
What is the difference between an endonuclease and an exonuclease?
An endonuclease cleaves nucleic acids internally at specific recognition sites, while an exonuclease cleaves nucleic acids at the ends by removing nucleotides one at a time. Endonucleases are involved in processes like DNA repair and recombination, while exonucleases are important for proofreading during DNA replication.
What is the difference between endonuclease and exonuclease activity in DNA degradation processes?
Endonuclease activity involves cutting DNA internally, while exonuclease activity involves cutting DNA from the ends. In DNA degradation processes, endonucleases break DNA strands at specific points, while exonucleases remove nucleotides from the ends of DNA strands.
What is is a linker?
The DNA found between nucleosomes on chromatin; since it is not complexed to proteins as strongly as other forms of dna, it is accessible to exonuclease hydrolysis.
What is the difference between DNA polymerase III and DNA polymerase I?
pol 1 - exonuclease activity pol 2 - dna repair pol 3 - primary replication enzyme
What are enzymes that have a proofreading role in DNA replication?
DNA polymerases, such as DNA polymerase III in prokaryotes and DNA polymerase delta in eukaryotes, have proofreading activities during DNA replication. These enzymes possess exonuclease activity, allowing them to detect and correct errors in newly synthesized DNA strands by removing misincorporated nucleotides. This proofreading function helps maintain the fidelity of DNA replication.
Polymerase 1 without 5' to 3' activity is called?
Polymerase 1 without 5' to 3' activity is called exonuclease. It is responsible for removing RNA primers during DNA replication.
How can one differentiate between single strand DNA and double strand DNA?
One can differentiate between single-strand DNA and double-strand DNA by treating with exonuclease I which specifically digests only ssDNA. Note. For verification, the products have to be run on polyacrylamide gels with appropriate controls. Hope this helps One can differentiate between single-strand DNA and double-strand DNA by treating with exonuclease I which specifically digests only ssDNA. Note. For verification, the products have to be run on polyacrylamide gels with appropriate controls. Hope this helps
Difference Between 3' to 5' and 5' to 3' Exonuclease Activity?
3'-5' is a characteristic feature of DNA-polymerase I. This activity is meant to repair any misparing mistakes that the enzyme may commit during the synthesis, in which the enzyme would reverse its direction by ONE NUCLEOTIDE and excised the mistakenly added nucleotide, the enzyme acts at the phosphodiester bond at the 5 prime. Whereas the 5'-3' exonuclease activity is an also repair strategy exercised by the DNA polymerase I. However, in this case the polymerase would move in the forwards direction and excise the miss-matched nucleotides at any position regardless with one nucleotide far or so many. This mechanism of repair is well documented in case UV-mutation.
