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The orcinol method for RNA estimation is based on the reaction of RNA with orcinol reagent in the presence of hydrochloric acid, which produces a green-colored complex. This reaction specifically targets ribose, a sugar component of RNA, leading to a color change that can be quantified spectrophotometrically. The intensity of the green color correlates with the RNA concentration, allowing for accurate quantification. This method is sensitive and can be used for various biological samples.
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