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The principle of the total hemolytic complement kit is based on the ability of complement proteins in serum to lyse antibody-sensitized red blood cells. When the serum is mixed with these sensitized cells, the complement system is activated, leading to the formation of the membrane attack complex that disrupts the cell membrane, resulting in hemolysis. The extent of hemolysis can be quantitatively measured, providing an indication of the total complement activity present in the serum sample. This test is useful for evaluating the complement system's functionality in various immunological and pathological conditions.

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3w ago

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The hemolytic complement kit is designed to assess the activity of the complement system, specifically its ability to lyse red blood cells (hemolysis). The principle involves mixing a sample containing complement proteins with sensitized red blood cells, which are coated with antibodies. If the complement system is functioning properly, it will activate and lead to the lysis of these red blood cells, resulting in a measurable change, typically a color change in the solution. This assay helps evaluate immune function and diagnose complement deficiencies or related diseases.


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