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How do you prepare 10mM solution of Tris-HCl?

To prepare a 10mM solution of Tris-HCl, you would weigh out the appropriate amount of Tris-HCl powder using a balance and dissolve it in water to make a final volume of solution. For example, to make 1L of 10mM Tris-HCl solution, you would need to dissolve 0.121g of Tris-HCl in 1L of water.


What Volume of 5M HCl to make tris buffer at pH 7.5?

The formula weight is 121.5 --> this is equivalent to 1M with 121.5g tris in 1L dH20. For a 5M stock, use 5x as much tris in the same 1L dh20.607.5 g tris into 800ml dH2O - stirring - then pH to 7.5 with 6M HCl and QS to your final volume of 1L


How do you prepare 10mM Tris Hcl?

1.21 g Tris-HCl, QS water to 1L. Scale appropriately.


750 ml of 20M HCl is mixed with 250 ml of 60M HCl What is the new molarity?

Molarity of a solution is the number of moles of the solute divided by the volume of the solution (in liters). If 750 ml of 20M HCl is mixed with 250 ml of 60M HCl, we first find the total number of moles of HCl in our new solution. Using that same formula, M=moles/V, we cansee that moles=MV. In the first solution we have (20M)(0.750L) = 15 moles. In the second, (60M)(0.250L) = 15 moles, so we have a total of 30 moles in our new solution, which also has a volume of 750mL + 250mL = 1L. The molarity of the new solution is 30 moles/1L = 30M


How many moles of HCL in 50ml of 1.0 M HCL?

50ml = .05L of HCL 1.0 M = 1mol / 1L of HCL simply multiply - .05 by 1.0, and get your answer!


How to prepare 1N HCl solution?

1N HCl is also 1M HCl because it is mono-protic. Therefore 36.5 g of HCl is required per liter or 3.65%. Simply take 100 g of 37% HCl and make up to the 1 liter mark on the volumetric flask. Check the value by titration against 1M NaOH. It should be perfect. If very slightly strong dilute very slightly (calculate) with water and re-standardize.


What is the volume of a cube with a surface area of 60cm2?

1000 cm3=1L


Is a liter a measure of mass?

no liter is a measurement of volume because 1L is 1000cm3 and cm3 is a measure of volume


How many moles of HCl in 25mL?

To determine the number of moles of HCl in 25mL, you need to know the concentration of the HCl solution. Once you have the concentration, you can use the formula: moles = volume (in liters) x concentration (in mol/L) to calculate the moles of HCl.


How is 1L of volume and 1kg of mass related to the meter?

They are all Metric measurements.


For measuring the volume of liquids?

you use nowadays... l ml 1000ml=1l


How do prepare 0.1m methanolic hcl?

To prepare 0.1 M methanolic HCl, you would first need to dilute concentrated HCl (usually in water) to the desired concentration (0.1 M) using methanol as the solvent. For example, if you have 1 M HCl, you would mix 1 volume of this with 9 volumes of methanol to obtain 0.1 M methanolic HCl. Care should be taken when handling concentrated HCl as it is corrosive and must be done in a fume hood due to the acidic vapors.