An equality sign
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The expression "Dang It" is usually used when someone is frustrated or annoyed - "Darn" or "Damn" could be another expression used.
substitution
Cloning vectors are used to increase the number of copies of the cloned gene or to amplify a foreign gene. Expression vectors are used to increase the expression of the foreign gene product.
A + B = C x = 2 + 6 It is an expression used to show an algebraic problem.
a phrase commonly used