Why 0.1ml of sample is used in spread plate rathar than 1 ml?

Answer 1

Using 0.1 ml of sample in a spread plate method helps to achieve more countable colonies. A smaller volume reduces the risk of overcrowding, allowing for better isolation and identification of individual colonies. This is particularly important for samples with high microbial density, where using 1 ml could result in too many colonies to count accurately. Additionally, using 0.1 ml ensures that the spread is more uniform across the agar surface.