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Incubating fermentation tubes beyond 24 hours can lead to over-fermentation, which may produce excessive amounts of byproducts such as alcohol and organic acids that can inhibit microbial growth or alter the desired fermentation process. Additionally, prolonged incubation may result in contamination from unwanted microorganisms, skewing results and compromising the integrity of the experiment. Maintaining a 24-hour limit helps ensure consistent and accurate fermentation measurements.

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Why are fermentation tubes evaluated at 24 and 48 hours?

It is important to remember that fermentation can occur with or without the presence of oxygen, and during prolonged incubation periods, many bacteria will begin growing oxidatively on the peptone after exhausting the supplied carbohydrate. This results in neutralization of the indicator, turning it black to red due to the alkaline conditions created by ammonia production. Consequently, fermentation tubes should not be incubated over 48 hours after inoculation for this reason.


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It is important because if you didn't, you would be measuring 12 hours instead of the full 24. I think...


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Why are fermentation tubes evaluated at 24 and 48 hours?

It is important to remember that fermentation can occur with or without the presence of oxygen, and during prolonged incubation periods, many bacteria will begin growing oxidatively on the peptone after exhausting the supplied carbohydrate. This results in neutralization of the indicator, turning it black to red due to the alkaline conditions created by ammonia production. Consequently, fermentation tubes should not be incubated over 48 hours after inoculation for this reason.


What dragon takes 48 hours to incubate?

in dragon city... legendary, pure and pure elemental...in dragonvale.... i don't know...


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