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The molecular sieving matrix is swelled prior to packing the column to enhance its porosity and improve the separation efficiency of the packed column. Swelling allows the matrix to expand, creating more uniform pores that facilitate the selective passage of molecules based on size. This pre-treatment also helps to eliminate air pockets and ensures a more consistent packing density, which is crucial for optimal chromatographic performance. Overall, swelling optimizes the interaction between the matrix and the analytes, leading to better resolution in separations.

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6d ago

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Q: Why is the molecular sieving matrix swelled prior to packing the column?
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