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Standards and samples must stand for 10 minutes before absorbance readings to allow for complete reaction stabilization and to ensure that any color development is consistent and uniform. This waiting period helps to minimize variations caused by factors such as temperature fluctuations or incomplete mixing. Additionally, it allows any transient changes in absorbance to settle, providing more accurate and reliable measurements.

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1mo ago

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Why must the standards and samples stand for 10 minutes before the absorbances are read?

This is to allow time for the particles in the solutions to become evenly distributed and stable.


Calibrated the spectrophotometer with?

a blank solution before starting the experiment to account for any background interference. This ensures accurate and reliable measurement of the absorbance values of the samples. It helps to establish a consistent baseline for the instrument to improve the precision of the results obtained.


Can a turbid sample be measured for absorbance in UV spectrophotometer?

Yes, turbidity can interfere with absorbance readings in a UV spectrophotometer by causing scattering of light. To accurately measure absorbance in a turbid sample, the turbidity would need to be reduced or removed before analysis, for example by centrifugation or filtration.


Why must to zeroing before start using spetrophotometer?

Zeroing a spectrophotometer before use is essential to establish a baseline measurement. This process accounts for any absorbance or transmittance caused by the solvent or the cuvette itself, ensuring that the readings reflect only the sample's properties. By zeroing, you eliminate systematic errors, allowing for accurate and reliable data when measuring the concentration of analytes in your samples.


What is the absorbance at 320 nm?

To determine the absorbance at 320 nm, you need to measure the light intensity before and after it passes through a sample at that wavelength. Absorbance (A) is calculated using the formula A = log10(I0/I), where I0 is the incident light intensity and I is the transmitted light intensity. The specific absorbance value will depend on the properties of the sample being tested. If you have a specific sample or context in mind, please provide more details for a more tailored response.


How do you get free food samples?

You can contact the manufacturer and ask if they have samples. I've done this before. There are also several web sites that catalog various free samples, for which you can apply.


How many minutes is it before noon if 70 minutes?

50 minutes before noon.


When do you not take a blood sample?

Samples must be take before milking


Before George Gallup which characteristic did polling samples often have?

biased


Why is it necessary to grind the food samples before testing?

ur face


10 minutes before 9?

10 minutes before nine is what


How many minutes is it before 12 if 20 minutes ago?

It would be 40 minutes before 12.